对感染瘙痒病的仓鼠大脑中瘙痒病相关纤维和朊病毒蛋白的超微结构研究。

Ultrastructural studies of scrapie-associated fibrils and prion protein from hamster brains infected with scrapie.

作者信息

Liberski P P, Płucienniczak A, Hrabec E, Bogucki A

机构信息

Department of Neurology, Academy of Medicine, Lódź, Poland.

出版信息

Folia Histochem Cytobiol. 1989;27(1):3-9.

PMID:2500370

Abstract

We report here about the purification of prion protein 27-30 (PrP 27-30) and scrapie-associated fibrils (SAF) from hamsters infected with the 263K strain of scrapie. Ultrastructural analysis of fractions from scrapie-infected brains revealed numerous fibrils measuring approximately 20 nm in diameter and 100-200 nm in length. The substructure of these fibrils consisted of protofilaments which were usually straight and rarely helically arranged. We conclude that the electron microscopic appearance of SAF depends much on the purification scheme.

摘要

我们在此报告从感染263K株羊瘙痒病的仓鼠中纯化朊病毒蛋白27 - 30（PrP 27 - 30）和羊瘙痒病相关纤维（SAF）的情况。对羊瘙痒病感染大脑的各组分进行超微结构分析，发现了许多直径约20纳米、长度为100 - 200纳米的纤维。这些纤维的亚结构由原纤维组成，原纤维通常是直的，很少呈螺旋排列。我们得出结论，SAF的电子显微镜外观很大程度上取决于纯化方案。