Rubenstein R, Merz P A, Kascsak R J, Scalici C L, Papini M C, Carp R I, Kimberlin R H
New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, Staten Island.
J Infect Dis. 1991 Jul;164(1):29-35. doi: 10.1093/infdis/164.1.29.
Scrapie-associated fibrils (SAF) and protease-resistant proteins (PrP) were isolated from spleens and brains of clinical animals (mice and hamsters) from three scrapie agent-host strain combinations, and their concentrations were compared with infectivity levels. The spleens of infected animals contained lower levels of infectivity, PrP, and SAF than did brains. Regardless of the route of infection, both SAF and infectivity were detected in spleen before brain. Infectivity increased in brains and spleens of 139A-infected mice before the detection and increase in SAF, suggesting that the synthesis of SAF and PrP may not be the limiting factor in agent replication. In contrast to those in ME7- and 263K-infected animals, the Western blot profiles for PrP from brain and spleen of 139A-infected mice exhibited distinct differences. Results indicate that SAF and PrP found in the spleens are both organ- and scrapie strain-specific.
从三种羊瘙痒病病原体-宿主毒株组合的临床发病动物（小鼠和仓鼠）的脾脏和大脑中分离出羊瘙痒病相关纤维（SAF）和抗蛋白酶蛋白（PrP），并将它们的浓度与感染性水平进行比较。感染动物的脾脏中感染性、PrP和SAF的水平低于大脑。无论感染途径如何，在大脑检测到SAF和感染性之前，脾脏中均可检测到SAF和感染性。在139A感染的小鼠中，大脑和脾脏中的感染性在SAF检测到并增加之前就已增加，这表明SAF和PrP的合成可能不是病原体复制的限制因素。与ME7和263K感染的动物不同，139A感染小鼠大脑和脾脏中PrP的蛋白质印迹图谱表现出明显差异。结果表明，在脾脏中发现的SAF和PrP具有器官和羊瘙痒病毒株特异性。
