Laboratory of Pharmacology and Toxicology, University of Yaoundé I, Yaoundé, Cameroon , Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, Ngaoundéré, Cameroon , Institute for Experimental Immunology and Hepatology, Center for Experimental Medicine, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
Am J Chin Med. 2014;42(4):853-68. doi: 10.1142/S0192415X14500542.
Entada africana (Ea) is a medicinal plant from the family of Fabaceae, used in Western and Central Africa regions to treat liver diseases. Antiviral properties of this plant were reported against Hepatitis B virus, while effects against Hepatitis C virus (HCV) remained unknown. This study reports for the first time, the effects of Ea crude extract and fractions on HCV replication. Furthermore, the effect of one Ea fraction on the transcriptional expression of two interferon-stimulated genes (ISGs) was also investigated. A methylene chloride-methanol (MCM) stem bark crude extract and different MCM fractions (EaF0, EaF5, EaF10, EaF25, and EaF100) were prepared and tested on LucUbiNeo-ET and Huh 5.15 cells lines used as genotype 1b (GT1b) replicon systems. The cells were incubated with crude extract and fractions at various concentrations. Then, the antiviral activity was assessed by luciferase reporter assay and the cell viability by MTT assay. Gene expression was also analyzed using quantitative real time RT-PCR. Results showed that the Ea crude extract dose-dependently inhibited HCV replication after 24 and 72 h of incubation. The MCM fraction (EaF10) exhibited the strongest anti-HCV properties with an IC50 = 0.453 ± 0.00117 mg/ml and no reduction of cell viability at antiviral concentrations. This fraction also significantly induced the expression of heme oxygenase-1 (HO-1) (5.36-fold), and 2'-5' oligoadenylate synthetase-3 (OAS-3) by 4.46-fold after 6 h and 2.31-fold after 24 h at the mRNA levels. Taken altogether, these results suggest that Ea may contain ingredients that indirectly regulate HCV replication.
非洲_entada（Ea）是豆科植物家族中的一种药用植物，在西非和中非地区用于治疗肝脏疾病。该植物具有抗乙型肝炎病毒的抗病毒特性，而对丙型肝炎病毒（HCV）的作用仍不清楚。本研究首次报道了 Ea 粗提取物和馏分对 HCV 复制的影响。此外，还研究了 Ea 馏分之一对两种干扰素刺激基因（ISGs）转录表达的影响。制备了二氯甲烷-甲醇（MCM）树皮粗提取物和不同的 MCM 馏分（EaF0、EaF5、EaF10、EaF25 和 EaF100），并在作为 1b 基因型（GT1b）复制子系统的 LucUbiNeo-ET 和 Huh 5.15 细胞系上进行了测试。用不同浓度的粗提取物和馏分孵育细胞。然后，通过荧光素酶报告基因测定法评估抗病毒活性，通过 MTT 测定法评估细胞活力。还使用定量实时 RT-PCR 分析基因表达。结果表明，Ea 粗提取物在孵育 24 和 72 小时后剂量依赖性地抑制 HCV 复制。MCM 馏分（EaF10）表现出最强的抗 HCV 特性，IC50=0.453±0.00117mg/ml，在抗病毒浓度下对细胞活力没有降低。该馏分还在 6 小时时使血红素加氧酶-1（HO-1）（5.36 倍）和 2'-5'寡聚腺苷酸合成酶-3（OAS-3）的表达显著诱导，mRNA 水平分别在 24 小时时诱导 4.46 倍和 2.31 倍。总的来说，这些结果表明 Ea 可能含有间接调节 HCV 复制的成分。
