College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea.
Am J Chin Med. 2014;42(4):1021-34. doi: 10.1142/S0192415X14500645.
Hair loss is accompanied by keratinocyte apoptosis-regression during catagen and prolonged telogen. Angelica sinensis was reported to promote hair growth in vitro. Based on previous studies, we explored the hair growth effect and the mechanism of A. sinensis related to keratinocyte apoptosis-regression during catagen in mice. The 70% Ethanol extract of A. sinensis was applied topically at doses of 1 and 100 mg/mL to the dorsa of C57BL/6 mice for 2 weeks. The A. sinensis-treated group showed noticeable hair regrowth. Treatment with A. sinensis restored the lengths of hair shafts and size of hair follicles. In addition, mice treated with A. sinensis showed notably decreased apoptotic cells, along with a significant change in the expression of cleaved caspase-3 and the ratio of a pair of apoptosis-associated proteins: Bcl-2 and Bax. Also, A. sinensis inhibited the nuclear translocation of NF-κB, the phosphorylation of IκB-α, the phosphorylation of three mitogen-activated protein MAP kinases, and the activation of c-Jun with decreased TNF-α. These findings reveal a role of A. sinensis as an alternative treatment for hair loss that acts through hair cycle pathways associated with apoptosis regression during catagen.
脱发伴随着退行期角质形成细胞的凋亡-抑制和休止期的延长。当归被报道具有体外促进毛发生长的作用。基于以前的研究，我们探索了当归在小鼠退行期中与角质形成细胞凋亡-抑制相关的毛发生长作用及其机制。当归的 70%乙醇提取物以 1 和 100mg/mL 的剂量局部应用于 C57BL/6 小鼠背部，持续 2 周。当归处理组显示出明显的毛发再生。当归处理恢复了毛干的长度和毛囊的大小。此外，用当归处理的小鼠表现出明显减少的凋亡细胞，以及凋亡相关蛋白：Bcl-2 和 Bax 的一对的表达发生显著变化：cleaved caspase-3。此外，当归抑制了 NF-κB 的核易位、IκB-α 的磷酸化、三种丝裂原激活的蛋白 MAP 激酶的磷酸化以及 c-Jun 的激活，并降低了 TNF-α。这些发现揭示了当归作为脱发替代治疗的作用，其通过与退行期中的凋亡抑制相关的毛发生长途径发挥作用。
