Amorepacific R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
Department of Dermatology, Dankook Medical College, Cheonan, Chungcheongnam-do 31116, Republic of Korea.
Int J Mol Med. 2017 Oct;40(4):1194-1200. doi: 10.3892/ijmm.2017.3107. Epub 2017 Aug 25.
It is well known that Panax ginseng (PG) has various pharmacological effects such as anti-aging and anti-inflammation. In a previous study, the authors identified that PG extract induced hair growth by means of a mechanism similar to that of minoxidil. In the present study, the inhibitory effect of PG extract on Dickkopf-1 (DKK-1)-induced catagen-like changes in hair follicles (HFs) was investigated in addition to the underlying mechanism of action. The effects of PG extract on cell proliferation, anti-apoptotic effect, and hair growth were observed using cultured outer root sheath (ORS) keratinocytes and human HFs with or without DKK-1 treatment. The PG extract significantly stimulated proliferation and inhibited apoptosis, respectively, in ORS keratinocytes. PG extract treatment affected the expression of apoptosis-related genes Bcl-2 and Bax. DKK-1 inhibited hair growth, and PG extract dramatically reversed the effect of DKK-1 on ex vivo human hair organ culture. PG extract antagonizes DKK-1-induced catagen-like changes, in part, through the regulation of apoptosis-related gene expression in HFs. These findings suggested that PG extract may reduce hair loss despite the presence of DKK-1, a strong catagen inducer via apoptosis.
众所周知，人参（PG）具有多种药理作用，如抗衰老和抗炎作用。在之前的研究中，作者发现 PG 提取物通过类似于米诺地尔的机制诱导头发生长。在本研究中，除了作用机制外，还研究了 PG 提取物对 Dickkopf-1（DKK-1）诱导的毛囊（HFs）退行期样变化的抑制作用。使用培养的外根鞘（ORS）角质形成细胞和有或没有 DKK-1 处理的人 HFs 观察 PG 提取物对细胞增殖、抗凋亡作用和毛发生长的影响。PG 提取物分别显着刺激 ORS 角质形成细胞的增殖和抑制凋亡。PG 提取物处理影响凋亡相关基因 Bcl-2 和 Bax 的表达。DKK-1 抑制毛发生长，PG 提取物显着逆转 DKK-1 对体外人毛器官培养的作用。PG 提取物通过调节 HFs 中凋亡相关基因的表达拮抗 DKK-1 诱导的退行期样变化。这些发现表明，PG 提取物可能会减少脱发，尽管存在 DKK-1，这是一种通过凋亡诱导强烈退行期的诱导剂。
