School of Science, Monash University, Jalan Lagoon Selatan, 46150, Bandar Sunway, Selangor, Malaysia.
Arch Microbiol. 2014 Oct;196(10):709-17. doi: 10.1007/s00203-014-1011-1. Epub 2014 Jul 9.
Nepenthes pitcher plants produce modified jug-shaped leaves to attract, trap and digest insect prey. We used 16S rDNA cloning and sequencing to compare bacterial communities in pitcher fluids of each of three species, namely Nepenthes ampullaria, Nepenthes gracilis and Nepenthes mirabilis, growing in the wild. In contrast to previous greenhouse-based studies, we found that both opened and unopened pitchers harbored bacterial DNA. Pitchers of N. mirabilis had higher bacterial diversity as compared to other Nepenthes species. The composition of the bacterial communities could be different between pitcher types for N. mirabilis (ANOSIM: R = 0.340, p < 0.05). Other Nepenthes species had similar bacterial composition between pitcher types. SIMPER showed that more than 50 % of the bacterial taxa identified from the open pitchers of N. mirabilis were not found in other groups. Our study suggests that bacteria in N. mirabilis are divided into native and nonnative groups.
猪笼草属植物会产生改良的壶状叶子来吸引、捕捉和消化昆虫猎物。我们使用 16S rDNA 克隆和测序技术比较了三种野生猪笼草（Nepenthes ampullaria、Nepenthes gracilis 和 Nepenthes mirabilis）的瓶内液体中的细菌群落。与之前基于温室的研究不同，我们发现无论是打开的还是未打开的瓶子都含有细菌 DNA。与其他猪笼草属物种相比，N. mirabilis 的细菌多样性更高。对于 N. mirabilis 来说，瓶型之间的细菌群落组成可能不同（ANOSIM：R = 0.340，p < 0.05）。其他猪笼草属物种的瓶型之间细菌组成相似。SIMPER 分析表明，从 N. mirabilis 敞口瓶中鉴定出的细菌分类群中，有超过 50%的在其他组中未发现。我们的研究表明，N. mirabilis 中的细菌分为本地和非本地两组。
