Departments of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
BMC Complement Altern Med. 2014 Jul 8;14:229. doi: 10.1186/1472-6882-14-229.
An overdose of the acetaminophen causes liver injury. This study aims to examine the anti-oxidative, anti-inflammatory effects of Aloe vera in mice with acetaminophen induced hepatitis.
Male mice were randomly divided into three groups (n = 8 each). Control group were given orally distilled water (DW). APAP group were given orally N-acetyl-P-aminophenol (APAP) 400 mg/kg suspended in DW. Aloe vera-treated group were given orally APAP and Aloe vera (150 mg/kg) suspended in DW. Twenty-four hours later, the liver was removed to determine hepatic malondialdehyde (MDA), hepatic glutathione (GSH), the number of interleukin (IL)-12 and IL-18 positive stained cells (%) by immunohistochemistry method, and histopathological examination. Then, the serum was collected to determine transaminase (ALT).
In APAP group, ALT, hepatic MDA and the number of IL-12 and IL-18 positive stained cells were significantly increased when compared to control group (1210.50 ± 533.86 vs 85.28 ± 28.27 U/L, 3.60 ± 1.50 vs 1.38 ± 0.15 nmol/mg protein, 12.18 ± 1.10 vs 1.84 ± 1.29%, and 13.26 ± 0.90 vs 2.54 ± 1.29%, P = 0.000, respectively), whereas hepatic GSH was significantly decreased when compared to control group (5.98 ± 0.30 vs 11.65 ± 0.43 nmol/mg protein, P = 0.000). The mean level of ALT, hepatic MDA, the number of IL-12 and IL-18 positive stained cells, and hepatic GSH in Aloe vera-treated group were improved as compared with APAP group (606.38 ± 495.45 vs 1210.50 ± 533.86 U/L, P = 0.024; 1.49 ± 0.64 vs 3.60 ± 1.50 nmol/mg protein, P = 0.001; 5.56 ± 1.25 vs 12.18 ± 1.10%, P = 0.000; 6.23 ± 0.94 vs 13.26 ± 0.90%, P = 0.000; and 10.02 ± 0.20 vs 5.98 ± 0.30 nmol/mg protein, P = 0.000, respectively). Moreover, in the APAP group, the liver showed extensive hemorrhagic hepatic necrosis at all zones while in Aloe vera-treated group, the liver architecture was improved histopathology.
APAP overdose can cause liver injury. Our result indicate that Aloe vera attenuate APAP-induced hepatitis through the improvement of liver histopathology by decreased oxidative stress, reduced liver injury, and restored hepatic GSH.
过量的对乙酰氨基酚会导致肝损伤。本研究旨在研究芦荟对乙酰氨基酚诱导的肝炎小鼠的抗氧化、抗炎作用。
雄性小鼠随机分为三组（每组 8 只）。对照组给予口服蒸馏水（DW）。APAP 组给予口服 N-乙酰-P-氨基酚（APAP）400mg/kg 悬浮在 DW 中。芦荟处理组给予口服 APAP 和芦荟（150mg/kg）悬浮在 DW 中。24 小时后，取出肝脏，通过免疫组织化学方法测定肝丙二醛（MDA）、肝谷胱甘肽（GSH）、白细胞介素（IL）-12 和 IL-18 阳性染色细胞数（%），并进行组织病理学检查。然后，收集血清以测定转氨酶（ALT）。
与对照组相比，APAP 组 ALT、肝 MDA 和 IL-12 和 IL-18 阳性染色细胞数显著增加（1210.50±533.86 vs 85.28±28.27U/L、3.60±1.50 vs 1.38±0.15nmol/mg 蛋白、12.18±1.10 vs 1.84±1.29%和 13.26±0.90 vs 2.54±1.29%，P=0.000），而肝 GSH 与对照组相比显著降低（5.98±0.30 vs 11.65±0.43nmol/mg 蛋白，P=0.000）。与 APAP 组相比，芦荟处理组的 ALT、肝 MDA、IL-12 和 IL-18 阳性染色细胞数和肝 GSH 水平均有所改善（606.38±495.45 vs 1210.50±533.86U/L，P=0.024；1.49±0.64 vs 3.60±1.50nmol/mg 蛋白，P=0.001；5.56±1.25 vs 12.18±1.10%，P=0.000；6.23±0.94 vs 13.26±0.90%，P=0.000；和 10.02±0.20 vs 5.98±0.30nmol/mg 蛋白，P=0.000）。此外，在 APAP 组中，肝脏各区域均显示广泛出血性肝坏死，而在芦荟处理组中，肝脏结构得到改善。
APAP 过量可导致肝损伤。我们的结果表明，芦荟通过降低氧化应激、减轻肝损伤和恢复肝 GSH 来减轻 APAP 诱导的肝炎。
