Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
World J Gastroenterol. 2013 Mar 28;19(12):1962-7. doi: 10.3748/wjg.v19.i12.1962.
To investigate whether curcumin could attenuate hepatitis in mice with paracetamol overdose.
Male mice were divided into four groups. Group 1 (control, n = 8); was fed with distilled water; Group 2 [N-acetyl-P-aminophenol (APAP), n = 8]; was fed with a single dose of 400 mg/kg APAP dissolved in distilled water; Group 3 [APAP + curcumin (CUR) 200, n = 8], was fed with a single dose of 400 mg/kg APAP and 200 mg/kg CUR; Group 4 (APAP + CUR 600, n = 8), was fed with a single dose of 400 mg/kg APAP and 600 mg/kg CUR. Twenty-four hours later, the liver was removed to examine hepatic glutathione (GSH), hepatic malondialdehyde (MDA), and histopathologically. Then whole blood was withdrawn from heart to determine transaminase (serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase) and inflammatory cytokines [interleukin (IL)-12 and IL-18] levels by enzyme linked immunosorbent assay.
Serum transaminase, hepatic MDA, and inflammatory cytokines increased significantly in the APAP compared with the control group. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups significantly decreased these parameters compared with the APAP group. The level of GSH decreased significantly in the APAP compared with the control group. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups significantly increased these parameters compared with the APAP group. The histological appearance of the liver in the control group showed normal. In the APAP-treated group, the liver showed extensive hemorrhagic hepatic necrosis at all zones. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups, caused the liver histopathology to improve. In the APAP + CUR 200 group, the liver showed focal necrosis and but the normal architecture was well preserved in APAP + CUR 600 group.
APAP overdose can cause liver injury. Results indicate that curcumin prevents APAP-induced hepatitis through the improvement of liver histopathology by decreased oxidative stress, reduced liver inflammation, and restoration of GSH.
研究姜黄素是否能减轻对乙酰氨基酚过量的小鼠肝炎。
雄性小鼠分为四组。第 1 组（对照组，n=8），用蒸馏水喂养；第 2 组（N-乙酰对氨基酚（APAP），n=8），用 400mg/kgAPAP 溶于蒸馏水的单次剂量喂养；第 3 组（APAP+姜黄素 200，n=8），用 400mg/kgAPAP 和 200mg/kgCUR 的单次剂量喂养；第 4 组（APAP+姜黄素 600，n=8），用 400mg/kgAPAP 和 600mg/kgCUR 的单次剂量喂养。24 小时后，取出肝脏检查肝谷胱甘肽（GSH）、肝丙二醛（MDA）和组织病理学。然后从心脏抽取全血，通过酶联免疫吸附法测定转氨酶（血清谷氨酸草酰乙酸转氨酶和血清谷氨酸丙酮酸转氨酶）和炎症细胞因子[白细胞介素（IL）-12 和 IL-18]水平。
与对照组相比，APAP 组血清转氨酶、肝 MDA 和炎症细胞因子显著升高。与 APAP 组相比，APAP+CUR 200 和 APAP+CUR 600 组补充姜黄素显著降低了这些参数。与对照组相比，APAP 组 GSH 水平显著降低。与 APAP 组相比，APAP+CUR 200 和 APAP+CUR 600 组补充姜黄素显著增加了这些参数。对照组肝脏组织学表现正常。在 APAP 治疗组，所有区域的肝脏均显示广泛出血性肝坏死。APAP+CUR 200 和 APAP+CUR 600 组补充姜黄素使肝组织病理学得到改善。在 APAP+CUR 200 组，肝脏显示局灶性坏死，但在 APAP+CUR 600 组，正常结构得到很好的保留。
APAP 过量可引起肝损伤。结果表明，姜黄素通过降低氧化应激、减少肝炎症和恢复 GSH 来预防 APAP 诱导的肝炎。
