Randall L P, Lemma F, Rogers J P, Cheney T E A, Powell L F, Teale C J
Animal Health and Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
Animal Health and Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
J Antimicrob Chemother. 2014 Nov;69(11):2947-50. doi: 10.1093/jac/dku258. Epub 2014 Jul 7.
To determine the prevalence and types of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occurring in pigs at slaughter in the UK in 2013.
Caecal samples from 637 pigs, sampled via a UK-wide monitoring programme in 2013, were enriched overnight in buffered peptone water, before plating to CHROMagar CTX and Oxoid Brilliance ESBL agar. Presumptive ESBL-producing E. coli from both media were tested for ESBL phenotype using MAST ESβL ID discs. Isolates with an ESBL phenotype were examined for the presence of blaCTX-M, blaOXA, blaSHV and blaTEM genes using a multiplex PCR. All blaCTX-M and blaSHV genes identified by PCR were sequenced.
A total of 23.4% (95% CI 19.2-27.6) of pigs were positive for ESBL-producing E. coli; 22% (95% CI 17.8-26.1) of the pigs carried E. coli producing CTX-M enzymes [comprising enzyme types 1 (18.7% of pigs), 3 (0.2%), 14 (0.5%), 15 (1.4%), 27 (0.5%), 32 (0.5%) and 55 (0.3%)] and 2.2% (95% CI 0.8-3.6) of the pigs carried E. coli producing SHV-12. Five pigs carried both CTX-M- and SHV-12-producing E. coli as different isolates. There were no statistically significant differences observed between the two medium types in terms of the proportions of each CTX-M enzyme type isolated.
In this UK study, 23.4% of pigs were found to be positive for ESBL-producing E. coli using selective culture media. The use of two different commercially available ESBL isolation media was found to improve the detection of ESBL-producing E. coli.
确定2013年英国屠宰猪中产超广谱β-内酰胺酶（ESBL）大肠杆菌的流行率和类型。
2013年通过全英监测计划采集的637头猪的盲肠样本，先在缓冲蛋白胨水中过夜增菌，然后接种到CHROMagar CTX和Oxoid Brilliance ESBL琼脂平板上。使用MAST ESβL ID药敏纸片对两种培养基上疑似产ESBL的大肠杆菌进行ESBL表型检测。对具有ESBL表型的分离株，采用多重PCR检测blaCTX-M、blaOXA、blaSHV和blaTEM基因的存在情况。对PCR鉴定出的所有blaCTX-M和blaSHV基因进行测序。
共有23.4%（95%可信区间19.2 - 27.6）的猪产ESBL大肠杆菌呈阳性；22%（95%可信区间17.8 - 26.1）的猪携带产CTX-M酶的大肠杆菌[包括酶型1（占猪的18.7%）、3（0.2%）、14（0.5%）、15（1.4%）、27（0.5%）、32（0.5%）和55（0.3%）]，2.2%（95%可信区间0.8 - 3.6）的猪携带产SHV-12的大肠杆菌。5头猪分别携带产CTX-M和产SHV-12的不同大肠杆菌分离株。在分离出的每种CTX-M酶型比例方面，两种培养基类型之间未观察到统计学显著差异。
在这项英国研究中，使用选择性培养基发现23.4%的猪产ESBL大肠杆菌呈阳性。发现使用两种不同的市售ESBL分离培养基可提高产ESBL大肠杆菌的检测率。
