Ashour Tariq Helal
Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, P.O. Box 7607, 7152 Makkah, Saudi Arabia.
ISRN Hematol. 2014 Mar 30;2014:764754. doi: 10.1155/2014/764754. eCollection 2014.
The preventative effect of caffeic acid phenyl ester (CAPE) against hematological, blood coagulation, and hepatorenal disturbances in cadmium (Cd) intoxication was investigated in rats. Male Wistar rats were randomly assigned into control group, Cd-group, and Cd + CAPE group. Cd intoxication was induced by intraperitoneal injection (i.p.) of CdCl2 (1 mg/kg/day) for 21 days, and CAPE was daily given (10 micromol/kg; i.p.) for also 21 days. The results showed that Cd intoxication impaired hepatorenal function and significantly prolonged prothrombin time and activated partial thromboplastin time and decreased fibrinogen level, red blood cells and platelets counts, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. Interestingly, all these hematological, blood coagulation, and hepatorenal deteriorations of Cd toxicity were significantly prevented by CAPE. Additionally, CAPE significantly reversed the significant decreases in levels of total reduced glutathione and superoxide dismutase and increases in levels of thiobarbituric acid reactive substances that were observed in the sera and liver and kidney homogenates of Cd group. It is concluded that CAPE is a promising compound that can counteract the hematological and blood coagulation disturbances, oxidative stress, and hepatorenal damages in Cd intoxication. However, further studies are crucially needed to improve this treatment in patients.
在大鼠中研究了咖啡酸苯乙酯（CAPE）对镉（Cd）中毒所致血液学、血液凝固及肝肾紊乱的预防作用。雄性Wistar大鼠被随机分为对照组、Cd组和Cd + CAPE组。通过腹腔注射（i.p.）CdCl2（1毫克/千克/天）诱导Cd中毒21天，同时每天腹腔注射CAPE（10微摩尔/千克），持续21天。结果显示，Cd中毒损害了肝肾功 能，显著延长了凝血酶原时间和活化部分凝血活酶时间，并降低了纤维蛋白原水平、红细胞和血小板计数、血红蛋白浓度、血细胞比容、平均红细胞体积、平均红细胞血红蛋白含量及平均红细胞血红蛋白浓度。有趣的是，CAPE显著预防了Cd毒性导致的所有这些血液学、血液凝固及肝肾损害。此外，CAPE显著逆转了Cd组血清、肝脏和肾脏匀浆中观察到的总还原型谷胱甘肽和超氧化物歧化酶水平的显著降低以及硫代巴比妥酸反应性物质水平的升高。结论是，CAPE是一种有前景的化合物，可对抗Cd中毒中的血液学和血液凝固紊乱、氧化应激及肝肾损害。然而，迫切需要进一步研究以改善对患者的这种治疗。
