Shivasharanappa Kirankumar, Londonkar Ramesh
Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
ISRN Pharmacol. 2014 Mar 31;2014:975303. doi: 10.1155/2014/975303. eCollection 2014.
The present study was carried out to investigate the thrombolytic and antimitotic potentiality of various extracts of fruits of Ficus glomerata, a traditional medicinal plant, using an in vitro assay method. Three crude extracts such as petroleum ether (FGPE), chloroform (FGCE), and methanol (FGME) were used for the study, with a standard (streptokinase) and negative control (sterile distilled water) to validate the method. The thrombolytic nature of the plant was found significant with methanol extract and chloroform and petroleum ether extracts have recorded mild activity, when compared with the negative control (sterile distilled water). The extracts have shown mild clot lysis, that is, 2.16%, 23.06%, 27.60%, and 47.74% of sterile distilled water, FGPE, FGCE, and FGME, respectively, while the standard (streptokinase) has shown 74.22% clot lysis. FGME inhibited the root growth in number as well as length effectively, followed by FGPE, while FGCE exhibited moderate antimitotic activity and it was supported by mitotic index. Therefore, the obtained results suggest that among all the extracts of plant the methanolic extract has shown highest thrombolytic and antimitotic activity.
本研究采用体外试验方法，对传统药用植物聚果榕果实的各种提取物的溶栓和抗有丝分裂潜力进行了研究。使用了三种粗提物，即石油醚提取物（FGPE）、氯仿提取物（FGCE）和甲醇提取物（FGME），并设置了标准品（链激酶）和阴性对照（无菌蒸馏水）来验证该方法。与阴性对照（无菌蒸馏水）相比，发现该植物的甲醇提取物具有显著的溶栓特性，氯仿提取物和石油醚提取物表现出轻微的活性。提取物表现出轻微的血凝块溶解，即分别为无菌蒸馏水、FGPE、FGCE和FGME的2.16%、23.06%、27.60%和47.74%，而标准品（链激酶）表现出74.22%的血凝块溶解。FGME有效地抑制了根的数量和长度的生长，其次是FGPE，而FGCE表现出中等的抗有丝分裂活性，这得到了有丝分裂指数的支持。因此，所得结果表明，在该植物的所有提取物中，甲醇提取物表现出最高的溶栓和抗有丝分裂活性。
