Popova Alexandra A, Koksharova Olga A, Lipasova Valentina A, Zaitseva Julia V, Katkova-Zhukotskaya Olga A, Eremina Svetlana Iu, Mironov Alexander S, Chernin Leonid S, Khmel Inessa A
Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russia.
Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russia ; M.V. Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Leninskie Gory 1-40, Moscow 119991, Russia.
Biomed Res Int. 2014;2014:125704. doi: 10.1155/2014/125704. Epub 2014 Jun 11.
In previous research, volatile organic compounds (VOCs) emitted by various bacteria into the chemosphere were suggested to play a significant role in the antagonistic interactions between microorganisms occupying the same ecological niche and between bacteria and target eukaryotes. Moreover, a number of volatiles released by bacteria were reported to suppress quorum-sensing cell-to-cell communication in bacteria, and to stimulate plant growth. Here, volatiles produced by Pseudomonas and Serratia strains isolated mainly from the soil or rhizosphere exhibited bacteriostatic action on phytopathogenic Agrobacterium tumefaciens and fungi and demonstrated a killing effect on cyanobacteria, flies (Drosophila melanogaster), and nematodes (Caenorhabditis elegans). VOCs emitted by the rhizospheric Pseudomonas chlororaphis strain 449 and by Serratia proteamaculans strain 94 isolated from spoiled meat were identified using gas chromatography-mass spectrometry analysis, and the effects of the main headspace compounds--ketones (2-nonanone, 2-heptanone, 2-undecanone) and dimethyl disulfide--were inhibitory toward the tested microorganisms, nematodes, and flies. The data confirmed the role of bacterial volatiles as important compounds involved in interactions between organisms under natural ecological conditions.
在先前的研究中，各种细菌排放到化学圈中的挥发性有机化合物（VOCs）被认为在占据相同生态位的微生物之间以及细菌与目标真核生物之间的拮抗相互作用中发挥着重要作用。此外，据报道，细菌释放的多种挥发物可抑制细菌群体感应的细胞间通讯，并刺激植物生长。在此，主要从土壤或根际分离出的假单胞菌属和沙雷氏菌属菌株产生的挥发物对植物病原性根癌土壤杆菌和真菌具有抑菌作用，并对蓝细菌、果蝇（黑腹果蝇）和线虫（秀丽隐杆线虫）具有杀灭作用。利用气相色谱 - 质谱分析鉴定了从变质肉中分离出的根际绿针假单胞菌菌株449和粘质沙雷氏菌菌株94释放的VOCs，主要顶空化合物——酮类（2 - 壬酮、2 - 庚酮、2 - 十一烷酮）和二甲基二硫醚——对受试微生物、线虫和果蝇具有抑制作用。这些数据证实了细菌挥发物在自然生态条件下作为参与生物体间相互作用的重要化合物的作用。
