Paavanen-Huhtala Sari, Kalichamy Karunambigai, Pessi Anna-Mari, Häkkilä Sirkku, Saarto Annika, Tuomela Marja, Andersson Maria A, Koskinen Päivi J
Department of Biology, University of Turku, FI-20500 Turku, Finland.
Aerobiology Unit, Biodiversity Unit of the University of Turku, FI-20500 Turku, Finland.
Pathogens. 2023 Jan 19;12(2):161. doi: 10.3390/pathogens12020161.
Bad indoor air quality due to toxins and other impurities can have a negative impact on human well-being, working capacity and health. Therefore, reliable methods to monitor the health risks associated with exposure to hazardous indoor air agents are needed. Here, we have used transgenic nematode strains carrying stress-responsive fluorescent reporters and evaluated their ability to sense fungal or chemical toxins, especially those that are present in moisture-damaged buildings. Liquid-based or airborne exposure of nematodes to mycotoxins, chemical agents or damaged building materials reproducibly resulted in time- and dose-dependent fluorescent responses, which could be quantitated by either microscopy or spectrometry. Thus, the nematodes present an easy, ethically acceptable and comprehensive in vivo model system to monitor the response of multicellular organisms to indoor air toxicity.
由于毒素和其他杂质导致的室内空气质量差会对人类健康、工作能力和健康产生负面影响。因此，需要可靠的方法来监测与接触有害室内空气介质相关的健康风险。在这里，我们使用了携带应激反应荧光报告基因的转基因线虫菌株，并评估了它们感知真菌或化学毒素的能力，特别是那些存在于受潮损坏建筑物中的毒素。线虫通过液体或空气接触霉菌毒素、化学试剂或受损建筑材料，可重复地产生时间和剂量依赖性荧光反应，这些反应可以通过显微镜或光谱法进行定量。因此，线虫提供了一个简单、符合伦理且全面的体内模型系统，用于监测多细胞生物对室内空气毒性的反应。
