Institute of Molecular Genetics of National Research Center "Kurchatov Institute", Kurchatov sq. 2, Moscow, 123182, Russia.
Department of Biotechnology, Mendeleev University of Chemical Technology of Russia, Moscow, 125480, Russia.
Folia Microbiol (Praha). 2023 Aug;68(4):617-626. doi: 10.1007/s12223-023-01038-y. Epub 2023 Feb 15.
Volatile compounds emitted by bacteria can play a significant role in interacting with microorganisms, plants, and other organisms. In this work, we studied the effect of total gaseous mixtures of organic as well as inorganic volatile compounds (VCs) and individual pure volatile organic compounds (VOCs: ketones 2-nonanone, 2-heptanone, 2-undecanone, a sulfur-containing compound dimethyl disulfide) synthesized by the rhizosphere Pseudomonas chlororaphis 449 and Serratia plymuthica IC1270 strains, the soil-borne strain P. fluorescens B-4117, and the spoiled meat isolate S. proteamaculans 94 strain on Arabidopsis thaliana plants (on growth and germination of seeds). We demonstrated that total mixtures of volatile compounds emitted by these strains grown on Luria-Bertani agar, Tryptone Soya Agar, and Potato Dextrose Agar media inhibited the A. thaliana growth. When studied bacteria grew on Murashige and Skoog (MS) agar medium, volatile mixtures produced by bacteria could stimulate the growth of plants. Volatile compounds of bacteria slowed down the germination of plant seeds; in the presence of volatile mixtures of P. fluorescens B-4117, the seeds did not germinate. Of the individual VOCs, 2-heptanone had the most potent inhibitory effect on seed germination. We also showed that the tested VOCs did not cause oxidative stress in Escherichia coli cells using specific lux-biosensors. VOCs reduced the expression of the lux operon from the promoters of the katG, oxyS, and soxS genes (whose products involved in the protection of cells from oxidative stress) caused by the action of hydrogen peroxide and paraquat, respectively.
细菌排放的挥发性化合物在与微生物、植物和其他生物相互作用方面可发挥重要作用。在这项工作中，我们研究了总气态混合有机和无机挥发性化合物（VCs）以及由根际假单胞菌 449 和粘质沙雷氏菌 IC1270 菌株、土壤传播菌株荧光假单胞菌 B-4117 和变质肉分离株 S. proteamaculans 94 菌株合成的单个纯挥发性有机化合物（VOCs：酮 2-壬酮、2-庚酮、2-十一酮、含硫化合物二甲基二硫）对拟南芥植物（种子生长和发芽）的影响。我们证明，这些菌株在 Luria-Bertani 琼脂、胰蛋白胨大豆琼脂和马铃薯葡萄糖琼脂培养基上生长时排放的总挥发性化合物混合物抑制了拟南芥的生长。当研究的细菌在 Murashige 和 Skoog（MS）琼脂培养基上生长时，细菌产生的挥发性混合物可以刺激植物的生长。细菌的挥发性化合物减缓了植物种子的发芽；在存在荧光假单胞菌 B-4117 的挥发性混合物的情况下，种子不会发芽。在单个 VOCs 中，2-庚酮对种子发芽的抑制作用最强。我们还表明，使用特定的 lux 生物传感器，测试的 VOCs 不会在大肠杆菌细胞中引起氧化应激。VOCs 降低了过氧化氢和百草枯分别作用下 katG、oxyS 和 soxS 基因启动子 lux 操纵子的表达（其产物涉及细胞免受氧化应激的保护）。
