在先天性巨结肠症中，微小RNA-195通过下调DIEXF影响细胞迁移和细胞增殖。
MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung's disease.
作者信息
Lei Hao, Tang Junwei, Li Hongxing, Zhang Hongwei, Lu Changgui, Chen Huan, Li Wei, Xia Yankai, Tang Weibing
机构信息
State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
出版信息
BMC Gastroenterol. 2014 Jul 9;14:123. doi: 10.1186/1471-230X-14-123.
BACKGROUND
Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR.
METHODS
In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro.
RESULTS
Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation.
CONCLUSIONS
Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF.
背景
先天性巨结肠（HSCR）是最常见的先天性肠道动力障碍。我们旨在研究miR-195在HSCR发病机制中的作用。
方法
在本研究中，我们检测了78例HSCR患者和66例非HSCR对照者结肠组织中miRNA、mRNA和蛋白质的表达水平。采用Transwell、细胞计数试剂盒-8（CCK-8）和流式细胞术检测miR-195在体外的功能作用。
结果
我们的结果显示，HSCR患者的miR-195表达水平显著高于对照组；同时检测到消化器官扩张因子（DIEXF）表达水平异常降低。miR-195水平升高可抑制细胞中DIEXF的水平，从而导致细胞迁移和增殖受损。
结论
miR-195的异常表达可能通过下调DIEXF水平参与HSCR的发病机制。
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