Taheri Shima, Baier Grit, Majewski Peter, Barton Mary, Förch Renate, Landfester Katharina, Vasilev Krasimir
School of Engineering, University of South Australia, Mawson Lakes, SA 5095 Australia.
Nanotechnology. 2014 Aug 1;25(30):305102. doi: 10.1088/0957-4484/25/30/305102. Epub 2014 Jul 10.
Infections associated with medical devices are a substantial healthcare problem. Consequently, there has been increasing research and technological efforts directed toward the development of coatings that are capable of preventing bacterial colonization of the device surface. Herein, we report on novel hybrid silver loaded poly(L-lactic acid) nanoparticles (PLLA-AgNPs) with narrowly distributed sizes (17 ± 3 nm) prepared using a combination of solvent evaporation and mini-emulsion technology. These particles were then immobilized onto solid surfaces premodified with a thin layer of allylamine plasma polymer (AApp). The antibacterial efficacy of the PLLA-AgNPs nanoparticles was studied in vitro against both gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. The minimal inhibitory concentration values against Staphylococcus epidermidis and Escherichia coli were 0.610 and 1.156 μg · mL(-1), respectively. The capacity of the prepared coatings to prevent bacterial surface colonization was assessed in the presence of Staphylococcus epidermidis, which is a strong biofilm former that causes substantial problems with medical device associated infections. The level of inhibition of bacterial growth was 98%. The substrate independent nature and the high antibacterial efficacy of coatings presented in this study may offer new alternatives for antibacterial coatings for medical devices.
与医疗设备相关的感染是一个重大的医疗保健问题。因此，针对开发能够防止细菌在设备表面定植的涂层，人们开展了越来越多的研究并付出了技术努力。在此，我们报道了一种新型的负载银的聚（L-乳酸）纳米颗粒（PLLA-AgNPs），其尺寸分布狭窄（17±3纳米），采用溶剂蒸发和微乳液技术相结合的方法制备而成。然后将这些颗粒固定在预先用烯丙胺等离子体聚合物（AApp）薄层改性的固体表面上。对PLLA-AgNPs纳米颗粒针对革兰氏阳性菌（表皮葡萄球菌）和革兰氏阴性菌（大肠杆菌）的体外抗菌效果进行了研究。对表皮葡萄球菌和大肠杆菌的最低抑菌浓度值分别为0.610和1.156μg·mL⁻¹。在表皮葡萄球菌存在的情况下评估了所制备涂层防止细菌在表面定植的能力，表皮葡萄球菌是一种强大的生物膜形成菌，会引发与医疗设备相关感染的重大问题。细菌生长的抑制水平为98%。本研究中涂层所呈现的与底物无关的性质和高抗菌效果可能为医疗设备的抗菌涂层提供新的选择。
