School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, Queensland, 4000, Australia.
CSIRO-QUT Joint Sustainable Materials and Devices Laboratory, Commonwealth Scientific and Industrial Research Organisation, P.O. Box 218, Lindfield, NSW, 2070, Australia.
Sci Rep. 2017 May 8;7(1):1591. doi: 10.1038/s41598-017-01669-5.
Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO-nAg nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely Gram-positive coccal Staphylococcus aureus and Gram-negative rod-shaped Escherichia coli and Proteus mirabilis are investigated. At the same concentration (100 µg/ml), rGO-nAg nanocomposite was significantly more effective against all three pathogens than either rGO or nAg. The nanocomposite was equally active against P. mirabilis and S. aureus as systemic antibiotic nitrofurantoin, and significantly more effective against E. coli. Importantly, the inhibition was much faster in the case of rGO-nAg nanocomposite compared to nitrofurantoin, attributed to the synergistic effects of rGO-nAg mediated contact killing and oxidative stress. This study may provide new insights for the better understanding of antibacterial actions of rGO-nAg nanocomposite and for the better designing of graphene-based antibiotics or other biomedical applications.
还原氧化石墨烯（rGO）是一种很有前途的抗菌材料，其抗菌效果可以通过添加银纳米粒子（nAg）进一步提高。在这项研究中，研究了 rGO-nAg 纳米复合材料对几种重要的人类致病多药耐药菌（即革兰阳性球菌金黄色葡萄球菌和革兰阴性杆菌大肠杆菌和奇异变形杆菌）的抗菌活性的作用机制。在相同浓度（100μg/ml）下，rGO-nAg 纳米复合材料对所有三种病原体的抗菌效果均明显优于 rGO 或 nAg。该纳米复合材料对奇异变形杆菌和金黄色葡萄球菌的活性与全身抗生素呋喃妥因相当，对大肠杆菌的活性明显更强。重要的是，与呋喃妥因相比，rGO-nAg 纳米复合材料的抑制作用更快，这归因于 rGO-nAg 介导的接触杀伤和氧化应激的协同作用。这项研究可能为更好地理解 rGO-nAg 纳米复合材料的抗菌作用以及更好地设计基于石墨烯的抗生素或其他生物医学应用提供新的见解。
