Dubový P, Soukup T
Department of Anatomy, Medical Faculty, Purkynĕ University Brno, Czechoslovakia.
Acta Histochem. 1989;85(2):195-202. doi: 10.1016/S0065-1281(89)80068-6.
Electron microscopical localization of non-specific cholinesterase (nChE) activity was studied in tendon organs of the rat hindlimb muscles. The comparison between neurotendinous part (with high nChE activity) and purely collagenous compartment(s) (with very low nChE activity) demonstrated that Schwann cells are the fundamental source of this enzyme in rat tendon organs. Although particles of the nChE reaction product were also found in and around fibroblasts in both neurotendinous and purely collagenous compartments, their contribution to the overall nChE was not significant. nChE activity in rat tendon organs displayed heterogeneity along the Ib sensory axon; the highest activity was related to the Schwann cell investment of the unmyelinated part of Ib axon, lower activity to sensory terminals covered only by basement membrane and negligible activity to the myelinated part of sensory axons. Particles of the non-specific cholinesterase reaction product persisted in the basement membrane of Schwann cells 20 d after degeneration of Ib sensory axons and their terminals. The function of non-specific cholinesterase in sensory receptors is still not clear. It is suggested that this enzyme may be involved in the maintenance of the ionic milieu around sensory axon terminals during or after functional activity.
对大鼠后肢肌肉腱器官中非特异性胆碱酯酶（nChE）活性进行了电子显微镜定位研究。对神经腱部分（nChE活性高）和纯胶原部分（nChE活性极低）进行比较表明，雪旺细胞是大鼠腱器官中该酶的主要来源。尽管在神经腱和纯胶原部分的成纤维细胞内及周围也发现了nChE反应产物颗粒，但它们对总体nChE的贡献并不显著。大鼠腱器官中的nChE活性沿Ib感觉轴突呈现异质性；最高活性与Ib轴突无髓鞘部分的雪旺细胞包绕有关，较低活性与仅被基底膜覆盖的感觉终末有关，而感觉轴突有髓鞘部分的活性可忽略不计。Ib感觉轴突及其终末变性20天后，非特异性胆碱酯酶反应产物颗粒仍存在于雪旺细胞的基底膜中。感觉受体中非特异性胆碱酯酶的功能仍不清楚。有人提出，该酶可能在功能活动期间或之后参与维持感觉轴突终末周围的离子环境。
