Dubový P, Rosario C M, Aldskogius H
Department of Anatomy, Medical Faculty, Masaryk University, Brno, Czechoslovakia.
Histochem J. 1993 Feb;25(2):112-8. doi: 10.1007/BF00157982.
In the present study we describe the application of the non-specific cholinesterase (nChE) histochemical method for the detection of encapsulated sensory nerve endings prior to immunofluorescence staining of the sensory nerve fibres. The nChE staining of Schwann-derived structures surrounding sensory terminals allowed us to identify unequivocally the sensory corpuscles in the skin and the muscle proprioceptors (muscle spindles and Golgi tendon organs) in longitudinal sections of muscle tissue. The nChE staining of sensory nerve endings and immunofluorescence-labelled nerve fibres and their terminals could be viewed and photographed in the same section using appropriate filters. Since nChE activity persists in terminal Schwann cells for a long time after loss of the sensory axons, this combined enzyme- and immunohistochemical approach is also useful for experimental studies involving denervation and re-innervation of sensory nerve endings.
在本研究中，我们描述了非特异性胆碱酯酶（nChE）组织化学方法在感觉神经纤维免疫荧光染色之前用于检测被囊感觉神经末梢的应用。感觉末梢周围雪旺氏细胞衍生结构的nChE染色使我们能够在肌肉组织纵切片中明确识别皮肤中的感觉小体和肌肉本体感受器（肌梭和高尔基腱器官）。使用适当的滤光片，可以在同一切片中观察和拍摄感觉神经末梢的nChE染色以及免疫荧光标记的神经纤维及其末梢。由于在感觉轴突丧失后，nChE活性在终末雪旺氏细胞中持续很长时间，这种酶组织化学和免疫组织化学相结合的方法也可用于涉及感觉神经末梢去神经支配和再支配的实验研究。
