评估GenoType MTBDR检测方法在检测结核分枝杆菌复合群分离株中利福平及异烟肼耐药性方面的应用。
Evaluation of the GenoType MTBDR assay for detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex isolates.
作者信息
Saglik I, Oz Y, Kiraz N
机构信息
Department of Microbiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
出版信息
Indian J Med Microbiol. 2014 Jul-Sep;32(3):318-22. doi: 10.4103/0255-0857.136587.
Detection of drug resistance plays a critical role in tuberculosis treatment. The aim of this study was to evaluate the performance of GenoType Mycobacteria Drug Resistance (MTBDR) assay (Hain Lifescience, Germany) and to compare it with radiometric BACTEC 460 TB system (Becton Dickinson, USA) for the detection of rifampicin (RIF) and isoniazid (INH) resistance in 84 Mycobacterium tuberculosis complex (MTBC) isolates. RIF resistance was identified in 6 of 7 (85.7%) isolates and INH resistance was identified in 8 of 14 (57.1%) isolates by the GenoType MTBDR assay. Compared with BACTEC system, the sensitivity, specificity, positive predictive value and negative predictive values were 85.7%, 98.7%, 85.7% and 98.7% for RIF resistance; and 57.1%, 100%, 100% and 92.1% for INH resistance, respectively. GenoType MTBDR assay is reliable when tested specimen is resistant to the tested drugs. Although test was more successful in the detection of RIF resistance, it exhibited low sensitivity for the detection of INH resistance.
耐药性检测在结核病治疗中起着关键作用。本研究的目的是评估Genotype分枝杆菌耐药性（MTBDR）检测方法（德国海因生命科学公司）的性能，并将其与放射性BACTEC 460结核系统（美国BD公司）进行比较，以检测84株结核分枝杆菌复合群（MTBC）分离株中的利福平（RIF）和异烟肼（INH）耐药性。通过Genotype MTBDR检测方法，在7株分离株中有6株（85.7%）鉴定出RIF耐药，在14株分离株中有8株（57.1%）鉴定出INH耐药。与BACTEC系统相比，RIF耐药的敏感性、特异性、阳性预测值和阴性预测值分别为85.7%、98.7%、85.7%和98.7%；INH耐药的分别为57.1%、100%、100%和92.1%。当检测标本对检测药物耐药时，Genotype MTBDR检测方法是可靠的。虽然该检测在RIF耐药检测方面更成功，但在INH耐药检测方面表现出低敏感性。
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