通过冷冻电子断层扫描确定埃博拉病毒糖蛋白粘蛋白样结构域的空间定位。
Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.
作者信息
Tran Erin E H, Simmons James A, Bartesaghi Alberto, Shoemaker Charles J, Nelson Elizabeth, White Judith M, Subramaniam Sriram
机构信息
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
出版信息
J Virol. 2014 Sep;88(18):10958-62. doi: 10.1128/JVI.00870-14. Epub 2014 Jul 9.
Abstract
The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.
摘要
埃博拉病毒糖蛋白粘蛋白样结构域（MLD）与埃博拉病毒进入细胞及免疫逃逸有关。通过对埃博拉病毒样颗粒进行冷冻电子断层扫描，我们确定了全长糖蛋白在近天然状态下的三维结构，并将其与缺乏MLD的糖蛋白结构进行比较。我们的结果表明，MLD位于每个糖蛋白单体的顶端和侧面，为分析MLD功能提供了结构模板。
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1
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2
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PLoS One. 2013;8(2):e56265. doi: 10.1371/journal.pone.0056265. Epub 2013 Feb 18.
3
Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques.植物源单克隆抗体延迟埃博拉病毒感染治疗可为恒河猴提供保护。
Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18030-5. doi: 10.1073/pnas.1213709109. Epub 2012 Oct 15.
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PLoS Pathog. 2012;8(7):e1002797. doi: 10.1371/journal.ppat.1002797. Epub 2012 Jul 12.
5
Successful treatment of ebola virus-infected cynomolgus macaques with monoclonal antibodies.用单克隆抗体成功治疗感染埃博拉病毒的食蟹猴。
Sci Transl Med. 2012 Jun 13;4(138):138ra81. doi: 10.1126/scitranslmed.3003876.
6
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EMBO J. 2012 Apr 18;31(8):1947-60. doi: 10.1038/emboj.2012.53. Epub 2012 Mar 6.
7
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PLoS One. 2012;7(1):e29608. doi: 10.1371/journal.pone.0029608. Epub 2012 Jan 11.
8
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9
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