Tran Erin E H, Simmons James A, Bartesaghi Alberto, Shoemaker Charles J, Nelson Elizabeth, White Judith M, Subramaniam Sriram
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
J Virol. 2014 Sep;88(18):10958-62. doi: 10.1128/JVI.00870-14. Epub 2014 Jul 9.
The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.
埃博拉病毒糖蛋白粘蛋白样结构域（MLD）与埃博拉病毒进入细胞及免疫逃逸有关。通过对埃博拉病毒样颗粒进行冷冻电子断层扫描，我们确定了全长糖蛋白在近天然状态下的三维结构，并将其与缺乏MLD的糖蛋白结构进行比较。我们的结果表明，MLD位于每个糖蛋白单体的顶端和侧面，为分析MLD功能提供了结构模板。
