Sethi S K
Acta Virol. 1978 Jan;22(1):60-5.
HeLa cell--agarose suspension method provides an excellent plaquing system for assay of rhinovirus serotypes which ordinarily produce indistinct plaques on cell monolayers. The variable plaque size of cloned virus progeny does not involve antigenic change in phenotype as shown by plaque neutralization test. The system has proved useful for accurate and reproducible plaque assay of rhinoviruses, assay of serum neutralizing antibodies and cloning of viruses. The plaques produced are large, clear and reproducible. The factors which influence the plaquing of rhinoviruses in this system are the age of the cells, cell density and pH of the medium.
HeLa细胞-琼脂糖悬浮法为鼻病毒血清型的检测提供了一种出色的蚀斑形成系统，这些血清型通常在细胞单层上产生不清晰的蚀斑。克隆病毒子代的蚀斑大小各异，但如蚀斑中和试验所示，其表型中不涉及抗原变化。该系统已被证明可用于鼻病毒的准确且可重复的蚀斑测定、血清中和抗体检测以及病毒克隆。所产生的蚀斑大、清晰且可重复。在该系统中影响鼻病毒蚀斑形成的因素包括细胞的年龄、细胞密度和培养基的pH值。
