Nates S V, Márquez A, Zapata M
Instituto de Virología, Dr. José M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Rev Argent Microbiol. 1986;18(1):1-6.
Under defined plaquing condition, Gilschrist strain of rubella virus showed different plaque morphology and plaque size when tested in Vero cell cultures. These differences were obtained by changing the fetal bovine serum (FBS) concentration. With 2 per cent of FBS rubella virus formed clear plaques that included a number of cells which retained the stain, while with 4 per cent of FBS it formed ring-shaped plaques. These characteristics were retained even after the Gilschrist strain was passaged several times in Vero cell cultures. The cytopathic effect in Vero cell cultures proved to be useful for the titration of rubella virus, giving infective titres in the same logarithmic order than the plaque assay.
在规定的蚀斑形成条件下，风疹病毒吉尔克里斯特毒株在Vero细胞培养物中进行检测时，表现出不同的蚀斑形态和蚀斑大小。这些差异是通过改变胎牛血清（FBS）浓度获得的。当FBS浓度为2%时，风疹病毒形成清晰的蚀斑，其中包括一些保留染色的细胞，而当FBS浓度为4%时，它形成环形蚀斑。即使吉尔克里斯特毒株在Vero细胞培养物中传代多次后，这些特征仍然保留。Vero细胞培养物中的细胞病变效应被证明可用于风疹病毒的滴定，其感染滴度与蚀斑测定法的对数顺序相同。
