Fiala M
Appl Microbiol. 1968 Oct;16(10):1445-50. doi: 10.1128/am.16.10.1445-1450.1968.
Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced plaques only in a sensitive clonal line isolated from the M HeLa line. Rhinovirus type 4 produced plaques after the pretreatment of cells with actinomycin D. Enhancement of plaque formation by MgCl(2) has been demonstrated to date with 17 rhinoviruses. Some rhinoviruses were enhanced either by DEAE-dextran or by dextran sulfate in the overlay. No inhibitors were found in any of 10 bovine sera tested. Rhinovirus type 2 was visualized inside HeLa cells by electron microscopy and there appeared to be a very high ratio of physical particles per infectious unit of virus.
报道了55种鼻病毒在几株HeLa细胞系中产生蚀斑的情况。49种类型在先前所述条件下产生肉眼可见的蚀斑，这些条件包括在覆盖物中使用30 mM MgCl₂和30 μg/ml的DEAE-葡聚糖以及M HeLa细胞。基本上观察到三种类型的蚀斑：清晰的大、中型蚀斑和小的浑浊蚀斑。5种鼻病毒仅在从M HeLa系分离出的敏感克隆系中产生蚀斑。4型鼻病毒在用放线菌素D预处理细胞后产生蚀斑。迄今为止，已证明17种鼻病毒可通过MgCl₂增强蚀斑形成。一些鼻病毒在覆盖物中可被DEAE-葡聚糖或硫酸葡聚糖增强。在所测试的10份牛血清中均未发现抑制剂。通过电子显微镜在HeLa细胞内观察到2型鼻病毒，并且病毒的每个感染单位的物理颗粒比例似乎非常高。
