Birk I M, Dierstein R, Kaiser I, Matern U, König W A, Krebber R, Weckesser J
Institut für Biologie II, Albert-Ludwigs-Universität, Freiburg, Federal Republic of Germany.
Arch Microbiol. 1989;151(5):411-5. doi: 10.1007/BF00416599.
Toxic and nontoxic peptides were isolated from the cyanobacterium Microcystis aeruginosa PCC 7806 by a procedure including extraction of cells with water-saturated 1-butanol, chromatography of the extract on silica gel plates and high performance liquid chromatography (HPLC) on Partisil-5. The toxin was shown to be only a minor constituent, being negatively charged and thus separable by electrophoresis, within the HPLC-purified fraction. It contained erythro-beta-methyl-D-Asp, D-Glu, D-Ala, L-Leu, and L-Arg known to be part of the Microcystis peptide-toxin with Mr 994. The major part of the HPLC-purified fraction was assigned, however, to a nontoxic peptide with a Mr of 956. Partial hydrolysis studies of the nontoxic peptide(s) revealed amino acid sequences composed of D-Glu, N-methyl-Phe, and 3,4-dehydro-Pro, aside from the common L-amino acids. Cyclic linkage in the nontoxic peptide(s) appears likely.
通过包括用水饱和的1-丁醇提取细胞、在硅胶板上对提取物进行色谱分析以及在Partisil-5上进行高效液相色谱（HPLC）在内的方法，从铜绿微囊藻PCC 7806中分离出有毒和无毒肽。毒素被证明只是HPLC纯化级分中的一种次要成分，带负电荷，因此可通过电泳分离。它含有已知是微囊藻肽毒素一部分的赤藓糖-β-甲基-D-天冬氨酸、D-谷氨酸、D-丙氨酸、L-亮氨酸和L-精氨酸，其分子量为994。然而，HPLC纯化级分的主要部分被确定为一种分子量为956的无毒肽。对无毒肽的部分水解研究表明，除了常见的L-氨基酸外，其氨基酸序列由D-谷氨酸、N-甲基苯丙氨酸和3,4-脱氢脯氨酸组成。无毒肽中似乎存在环状连接。
