Watanabe M F, Oishi S
Appl Environ Microbiol. 1982 Apr;43(4):819-22. doi: 10.1128/aem.43.4.819-822.1982.
A toxic substance contained in the blue-green alga Microcystis aeruginosa was purified and partially characterized. Toxic algal cells were collected from a highly eutrophic lake in Japan, and the toxin was purified by homogenization, ultrafiltration, gel filtration, and ion-exchange chromatography. The final preparation gave a single peak on high-performance liquid chromatography. The toxicity was somewhat less than that reported for other toxins from this alga. The water extract of 6.7 mg (dry weight) of cells and 72 microgram of the purified protein was required to kill a mouse (1 mouse unit). The main amino acids of the toxin were glutamic acid, asparatic acid, alanine, glycine, arginine, and leucine. The molecular weight of the toxin was 2,950 as determined by high-performance liquid chromatography.
对铜绿微囊藻中所含的一种有毒物质进行了纯化并对其部分特性进行了表征。从日本一个高度富营养化的湖泊中收集有毒藻类细胞，通过匀浆、超滤、凝胶过滤和离子交换色谱法对毒素进行纯化。最终制剂在高效液相色谱上呈现单一峰。其毒性略低于报道的该藻类其他毒素的毒性。杀死一只小鼠（1个小鼠单位）需要6.7毫克（干重）细胞的水提取物和72微克纯化蛋白。毒素的主要氨基酸为谷氨酸、天冬氨酸、丙氨酸、甘氨酸、精氨酸和亮氨酸。通过高效液相色谱法测定，毒素的分子量为2950。
