Eriksson J E, Hägerstrand H, Isomaa B
Department of Biology, Abo Akademi, Turku, Finland.
Biochim Biophys Acta. 1987 Oct 1;930(3):304-10. doi: 10.1016/0167-4889(87)90003-6.
The effects of a cyclic peptide toxin, isolated from the cyanobacterium Microcystis aeruginosa, on cell morphology and ion transport in human erythrocytes, isolated rat hepatocytes and mouse fibroblasts (3T3) were studied. Neither in erythrocytes nor in fibroblasts did the toxin cause morphological alterations. In hepatocytes the toxin induced marked morphological alterations at a concentration of about 50 nM. In erythrocytes and fibroblasts no effects on ion transport were observed. In hepatocytes the toxin induced a significant increase in both phosphate and potassium efflux at concentrations far below the concentration causing morphological alterations (0.1 and 1 nM, respectively). It is suggested that the cytotoxicity of the toxin is not due to a non-specific interaction with the plasma membrane and that the effects of the toxin in hepatocytes are probably due to an interaction of the toxin with cytoskeletal elements.
研究了从铜绿微囊藻中分离出的一种环肽毒素对人红细胞、分离的大鼠肝细胞和小鼠成纤维细胞（3T3）的细胞形态和离子转运的影响。该毒素在红细胞和成纤维细胞中均未引起形态学改变。在肝细胞中，该毒素在浓度约为50 nM时诱导明显的形态学改变。在红细胞和成纤维细胞中未观察到对离子转运的影响。在肝细胞中，该毒素在远低于引起形态学改变的浓度（分别为0.1和1 nM）时，诱导磷酸盐和钾外流显著增加。提示该毒素的细胞毒性并非由于与质膜的非特异性相互作用，且该毒素在肝细胞中的作用可能是由于毒素与细胞骨架成分的相互作用。
