Lu Yao, Li Chun-Yu, Lin Shu-Sen, Yuan Peng
Department of Anorectal, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China.
Exp Ther Med. 2014 Aug;8(2):607-613. doi: 10.3892/etm.2014.1736. Epub 2014 May 28.
The aim of the present meta-analysis was to evaluate the correlation between a common polymorphism, rs13361189 C>T in the immunity-related GTPase M () gene, and susceptibility to Crohn's disease (CD). The PubMed, CISCOM, CINAHL, Web of Science, Google Scholar, EBSCO, Cochrane Library and CBM databases were investigated from database inception through to October 1, 2013 without the application of any language restrictions. The meta-analysis was performed using STATA 12.0 software and the relative risk (RR) with a 95% confidence interval (CI) was calculated. Seven case-control studies were included with a total of 3,093 CD patients and 3,227 healthy control subjects. The results of the meta-analysis revealed that the rs13361189 polymorphism correlates with an increased risk of CD (T allele versus C allele: RR=1.25 with 95% CI, 1.04-1.50; P=0.016 and CT + TT versus CC: RR=1.21 with 95% CI, 1.03-1.42; P=0.018). A subgroup analysis conducted using a genotyping method indicated that the rs13361189 polymorphism was correlated with an increased risk of CD in the TaqMan (T allele versus C allele: RR=1.32 with 95% CI, 1.01-1.73; P=0.042) and the polymerase chain reaction-restriction fragment length polymorphism subgroups (T allele versus C allele: RR=1.80 with 95% CI, 1.32-2.45; P<0.001 and CT + TT versus CC: RR=1.61 with 95% CI, 1.19-2.18; P=0.018). However, no correlation was observed in the direct sequencing subgroup (P>0.05). Further subgroup analysis by sample size demonstrated significant correlations between the rs13361189 polymorphism and an increased risk of CD in the large sample-size subgroup (T allele versus C allele: RR=1.46 with 95% CI, 1.26-1.68; P<0.001 and CT + TT versus CC: RR=1.40 with 95% CI, 1.21-1.62; P<0.001). However, no correlation was identified between the rs13361189 polymorphism and CD risk in the small sample-size subgroup (P>0.05). The present meta-analysis indicated that the rs13361189 polymorphism may contribute to susceptibility to CD. Thus, rs13361189 polymorphism may be a potential biomarker for the early diagnosis of CD.
本荟萃分析的目的是评估免疫相关GTP酶M（）基因中常见的rs13361189 C>T多态性与克罗恩病（CD）易感性之间的相关性。对PubMed、CISCOM、CINAHL、Web of Science、谷歌学术、EBSCO、Cochrane图书馆和中国生物医学文献数据库进行了检索，检索时间从数据库建立至2013年10月1日，不设任何语言限制。使用STATA 12.0软件进行荟萃分析，并计算相对风险（RR）及95%置信区间（CI）。纳入了7项病例对照研究，共3093例CD患者和3227例健康对照者。荟萃分析结果显示，rs13361189多态性与CD风险增加相关（T等位基因与C等位基因：RR = 1.25，95%CI为1.04 - 1.50；P = 0.016；CT + TT与CC：RR = 1.21，95%CI为1.03 - 1.42；P = 0.018）。采用基因分型方法进行的亚组分析表明，rs13361189多态性与TaqMan亚组（T等位基因与C等位基因：RR = 1.32，95%CI为1.01 - 1.73；P = 0.042）及聚合酶链反应-限制性片段长度多态性亚组（T等位基因与C等位基因：RR = 1.80，95%CI为1.32 - 2.45；P < 0.001；CT + TT与CC：RR = 1.61，95%CI为1.19 - 2.18；P = 0.018）中的CD风险增加相关。然而，直接测序亚组未观察到相关性（P > 0.05）。按样本量进行进一步亚组分析表明，rs13361189多态性与大样本量亚组中的CD风险增加显著相关（T等位基因与C等位基因：RR = 1.46，95%CI为1.26 - 1.68；P < 0.001；CT + TT与CC：RR = 1.40，95%CI为1.21 - 1.62；P < 0.001）。然而，小样本量亚组中未发现rs13361189多态性与CD风险之间存在相关性（P > 0.05）。本荟萃分析表明，rs13361189多态性可能与CD易感性有关。因此，rs13361189多态性可能是CD早期诊断的潜在生物标志物。
