猪巨细胞病毒的基因组组织与分子特征。

Genomic organization and molecular characterization of porcine cytomegalovirus.

机构信息

Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China.

出版信息

Virology. 2014 Jul;460-461:165-72. doi: 10.1016/j.virol.2014.05.014. Epub 2014 Jun 4.

DOI:10.1016/j.virol.2014.05.014

PMID:25010282

Abstract

A strain of the porcine cytomegalovirus (PCMV), which causes inclusion body rhinitis in newborn piglets, has been characterized with respect to its complete genome sequence. The virus genome is 128,367bp, containing 79 predicted open reading frames (ORFs). Of these ORFs, 69 have counterparts in human herpesvirus 6A (HHV-6A), 6B (HHV-6B) and 7 (HHV-7), and two ORFs are homologous to other members in the subfamily Betaherpesvirinae. Eight ORFs have no homologs in herpesvirus. Homologs had higher identity and possessed similar orientation and location as roseoloviruses. The PCMV genome is a DR-U-DR type, similar to HHV-6A, HHV-6B and HHV-7, but the PCMV DR is shorter and lacks predicted genes and telomere-like sequences. Phylogenetic analyses of several core genes indicate that PCMV could be clustered in a branch with roseoloviruses. We suggest that PCMV could be classified as a member of the genus Roseolovirus of the subfamily Betaherpesvirinae.

摘要

一株引起新生仔猪包涵体鼻炎的猪巨细胞病毒（PCMV）的全基因组序列特征已被阐明。该病毒基因组全长 128367bp，包含 79 个预测开放阅读框（ORF）。其中 69 个 ORF 在人类疱疹病毒 6A（HHV-6A）、6B（HHV-6B）和 7（HHV-7）中有对应物，2 个 ORF 与β疱疹病毒亚科的其他成员同源。8 个 ORF 在疱疹病毒中没有同源物。同源物具有更高的同一性，并具有与玫瑰疱疹病毒相似的方向和位置。PCMV 基因组为 DR-U-DR 型，与 HHV-6A、HHV-6B 和 HHV-7 相似，但 PCMV 的 DR 较短，缺乏预测基因和端粒样序列。几个核心基因的系统发育分析表明，PCMV 可能与玫瑰疱疹病毒聚类在一个分支中。我们建议将 PCMV 归类为β疱疹病毒亚科玫瑰疱疹病毒属的一个成员。