Goltz M, Widen F, Banks M, Belak S, Ehlers B
Robert Koch-Institut, Berlin, Germany.
Virus Genes. 2000 Oct;21(3):249-55. doi: 10.1023/a:1008156017758.
Porcine cytomegalovirus (PCMV) is an undesired pathogen in pigs intended for use as organ donors in xenotransplantation. In the present work, we characterized the first set of genes of PCMV. From a German isolate, the DNA polymerase (DPOL) locus was amplified and two complete open reading frames (ORF) as well as two partial ORFs including the complete DPOL gene and the 3'-end of the glycoprotein gB gene were sequenced. The deduced amino acid sequences showed the highest identities with the respective proteins of the betaherpesviruses, in particular those (ORFs 36-39) of the human herpesviruses 6 and 7 (HHV-6 and -7). In phylogenetic analysis, PCMV clustered also with HHV-6 and HHV-7. On this basis, PCMV could be firmly classified to the Betaherpesvirinae and tentatively assigned to the genus Roseolovirus. In addition to the German isolate, the DPOL gene was analysed from a British and a Japanese strain as well as a Spanish isolate. Differences of 0.4 to 1% were found on the nucleotide and the amino acid level. On the basis of the conserved regions, primer pairs were selected for PCR which detected PCMV in blood and tissue samples from four European countries. Therefore, these are the first nucleic acid-based test systems which were shown to universally detect PCMV. The application of these assays to organs of domestic pigs from Germany revealed a PCMV prevalence of > 50%.
猪巨细胞病毒（PCMV）是异种移植中用作器官供体的猪体内一种不良病原体。在本研究中，我们对PCMV的首批基因进行了特征分析。从一株德国分离株中扩增出DNA聚合酶（DPOL）基因座，并对两个完整的开放阅读框（ORF）以及两个部分ORF进行了测序，其中包括完整的DPOL基因和糖蛋白gB基因的3'端。推导的氨基酸序列与β疱疹病毒的相应蛋白具有最高的同源性，特别是与人类疱疹病毒6型和7型（HHV-6和HHV-7）的那些蛋白（ORF 36 - 39）。在系统发育分析中，PCMV也与HHV-6和HHV-7聚类。基于此，PCMV可被明确归类到β疱疹病毒亚科，并暂定归入玫瑰疹病毒属。除了德国分离株外，还对一株英国菌株、一株日本菌株以及一株西班牙分离株的DPOL基因进行了分析。在核苷酸和氨基酸水平上发现了0.4%至1%的差异。基于保守区域，选择了引物对用于PCR，该方法可检测来自四个欧洲国家的血液和组织样本中的PCMV。因此，这些是首批被证明能普遍检测PCMV的基于核酸的检测系统。将这些检测方法应用于来自德国的家猪器官，结果显示PCMV的流行率> 50%。
