基因组编辑工具包：靶向修饰的多种方法。
The genome editing toolbox: a spectrum of approaches for targeted modification.
机构信息
McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton Street, Stop C0400, Austin, TX 78712, USA.
McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton Street, Stop C0400, Austin, TX 78712, USA; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, USA.
出版信息
Curr Opin Biotechnol. 2014 Dec;30:87-94. doi: 10.1016/j.copbio.2014.06.005. Epub 2014 Jul 8.
The increase in quality, quantity, and complexity of recombinant products heavily drives the need to predictably engineer model and complex (mammalian) cell systems. However, until recently, limited tools offered the ability to precisely manipulate their genomes, thus impeding the full potential of rational cell line development processes. Targeted genome editing can combine the advances in synthetic and systems biology with current cellular hosts to further push productivity and expand the product repertoire. This review highlights recent advances in targeted genome editing techniques, discussing some of their capabilities and limitations and their potential to aid advances in pharmaceutical biotechnology.
重组产品的质量、数量和复杂性的增加，极大地推动了对可预测地工程改造模型和复杂（哺乳动物）细胞系统的需求。然而，直到最近，有限的工具提供了精确操纵其基因组的能力，从而阻碍了理性细胞系开发过程的全部潜力。靶向基因组编辑可以将合成生物学和系统生物学的进展与当前的细胞宿主相结合，进一步提高生产力并扩大产品种类。本文综述了靶向基因组编辑技术的最新进展，讨论了它们的一些功能和局限性，以及它们在促进药物生物技术发展方面的潜力。
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