Liu Dong, Pan Luqing, Li Zhen, Cai Yuefeng, Miao Jingjing
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
Ecotoxicol Environ Saf. 2014 Sep;107:251-9. doi: 10.1016/j.ecoenv.2014.06.024. Epub 2014 Jul 8.
A study was performed on clams (Ruditapes philippinarum) exposed to 0.03, 0.3 and 3μg/L benzo[a]pyrene (B[a]P) for 21 days. B[a]P metabolite contents, activities of aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH), dihydrodiol dehydrogenase (DD), glutathione-S-transferase (GST), sulfotransferase (SULT) and uridinediphosphate glucuronyltransferase (UGT) and B[a]P bioaccumulation were assayed in gills and digestive glands. Results showed that the order of B[a]P phase I metabolite contents was 9-hydroxy-B[a]P>B[a]P-1,6-dione>B[a]P-7,8-dihydrodiol, and the concentration of B[a]P-7,8-dihydrodiol sulfate conjugates was higher than that of B[a]P-7,8-dihydrodiol glucuronide conjugates. B[a]P accumulation and the activities of AHH, EROD, EH, DD, SULT and UGT increased first and then reached equilibrium. GST activity was induced first and then depressed. The concentration of B[a]P was far higher than that of its metabolites. Besides, there were no significant differences between enzyme activities in gills and those in digestive glands. These results provided information on B[a]P metabolic mechanism in bivalve and scientific data for pollution monitoring and food security.
对菲律宾蛤仔暴露于0.03、0.3和3μg/L苯并[a]芘（B[a]P）21天进行了一项研究。测定了鳃和消化腺中B[a]P代谢物含量、芳烃羟化酶（AHH）、7-乙氧基异吩恶唑酮O-脱乙基酶（EROD）、环氧化物水解酶（EH）、二氢二醇脱氢酶（DD）、谷胱甘肽-S-转移酶（GST）、磺基转移酶（SULT）和尿苷二磷酸葡萄糖醛酸基转移酶（UGT）的活性以及B[a]P生物累积情况。结果表明，B[a]P I相代谢物含量顺序为9-羟基-B[a]P>B[a]P-1,6-二酮>B[a]P-7,8-二氢二醇，且B[a]P-7,8-二氢二醇硫酸酯共轭物浓度高于B[a]P-7,8-二氢二醇葡萄糖醛酸酯共轭物浓度。B[a]P累积以及AHH、EROD、EH、DD、SULT和UGT的活性先升高然后达到平衡。GST活性先被诱导然后受到抑制。B[a]P的浓度远高于其代谢物浓度。此外，鳃和消化腺中的酶活性之间没有显著差异。这些结果提供了双壳贝类中B[a]P代谢机制的信息以及污染监测和食品安全的科学数据。
