Forriol Francisco, Longo Umile Giuseppe, Duart Julio, Ripalda Purification, Vaquero Javier, Loppini Mattia, Romeo Giovanni, Campi Stefano, Khan Wasim S, Muda Andrea O, Denaro Vincenzo
University Campus Bio- Medico of Rome, Italy.
Curr Stem Cell Res Ther. 2015;10(1):69-76. doi: 10.2174/1574888x09666140710115356.
To evaluate the effects of VEGF, BMP-7, Matrigel(TM), hyaluronic acid, in vitro cultured chondrocytes and trephination to promote and enhance the healing process of avascular meniscal tears in an animal model.
A longitudinal tear was produced in the inner avascular part of the meniscus of 24 sheeps. Each tear was treated with trephination technique and suture. The animals were divided into 6 groups to receive a different treatment: control (I); VEGF, BMP-7, Matrigel(TM), hyaluronic acid, in vitro cultured chondrocytes. At 8 weeks from surgery, meniscal samples were explanted and analyzed by histology, immunohistochemistry, and histomorphometry.
At the histological examination, Group IV and VI showed a partial closure of the meniscal lesion, whereas Group I, II, III, and V did not show any evidence of healing. In the group IV, the healed tissue represented the 22.95% of the lesion area. In the group VI, the healed tissue represented the 43.75% of the lesion area.
Autologous chondrocytes and BMP-7 associated with trephination and suture techniques enhanced healing process of meniscal tears in the avascular inner third of the meniscus in ovine model.
在动物模型中评估血管内皮生长因子（VEGF）、骨形态发生蛋白-7（BMP-7）、基质胶（Matrigel™）、透明质酸、体外培养的软骨细胞以及钻孔术对促进和增强半月板无血管区撕裂愈合过程的作用。
在24只绵羊半月板的无血管内侧部分制造一个纵向撕裂口。每个撕裂口采用钻孔技术并进行缝合处理。将动物分为6组，分别接受不同的治疗：对照组（I）；VEGF、BMP-7、基质胶（Matrigel™）、透明质酸、体外培养的软骨细胞。术后8周，取出半月板样本，通过组织学、免疫组织化学和组织形态计量学进行分析。
组织学检查显示，IV组和VI组半月板损伤有部分闭合，而I组、II组、III组和V组未显示任何愈合迹象。IV组中，愈合组织占损伤面积的22.95%。VI组中，愈合组织占损伤面积的43.75%。
在绵羊模型中，自体软骨细胞和BMP-7联合钻孔术和缝合技术可增强半月板无血管内侧三分之一区域撕裂的愈合过程。
