Xiao Hui, Wang Juan, Liu Yanan, Li Li
Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
Oncol Lett. 2014 Aug;8(2):582-588. doi: 10.3892/ol.2014.2173. Epub 2014 May 23.
c-Kit and epidermal growth factor receptor (EGFR) have critical roles in cell proliferation and differentiation in patients with non-small cell lung cancer (NSCLC). The present study aimed to investigate the prognostic impact of c-Kit and/or EGFR expression in tumor tissue samples from 146 patients with NSCLC. c-Kit expression was analyzed using immunohistochemistry and the expression of EGFR was assessed using fluorescence hybridization. Univariate and multivariate analyses identified that c-Kit is a significant negative prognostic factor. The expression of c-Kit was correlated with poor differentiation, pleura involvement and smoking history (P=0.043, 0.007 and 0.032, respectively). Furthermore, patients with c-Kit-positive expression were associated with a significantly lower overall survival compared with those exhibiting c-Kit-negative expression (P=0.048). The median follow-up time was 19 months post-surgery. EGFR gene amplification as a result of polysomy of chromosome 7 was found to be negatively correlated with poor differentiation and smoking history (P=0.023 and 0.044, respectively). The findings of the present study indicate that c-Kit and EGFR expression is a strong, independent, negative prognostic factor in NSCLC.
c-Kit和表皮生长因子受体（EGFR）在非小细胞肺癌（NSCLC）患者的细胞增殖和分化中起关键作用。本研究旨在调查c-Kit和/或EGFR表达对146例NSCLC患者肿瘤组织样本的预后影响。采用免疫组织化学分析c-Kit表达，使用荧光杂交评估EGFR表达。单因素和多因素分析确定c-Kit是一个显著的负性预后因素。c-Kit的表达与低分化、胸膜受累和吸烟史相关（分别为P = 0.043、0.007和0.032）。此外，与c-Kit阴性表达患者相比，c-Kit阳性表达患者的总生存期显著更低（P = 0.048）。术后中位随访时间为19个月。发现7号染色体多体导致的EGFR基因扩增与低分化和吸烟史呈负相关（分别为P = 0.023和0.044）。本研究结果表明，c-Kit和EGFR表达是NSCLC中一个强大的、独立的负性预后因素。
