Wang Xiangwei, Yang Liqun, Choi Jeong-Hyeon, Kitamura Eiko, Chang Chang-Sheng, Ding Jane, Lee Eun J, Cui Hongjuan, Ding Han-Fei
Genom Data. 2014 Dec 1;2:50-52. doi: 10.1016/j.gdata.2014.04.002.
Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in details the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication [1].
诱导分化是神经母细胞瘤（一种常见的小儿交感神经系统癌症）的一种治疗策略。同源盒蛋白HOXC9是神经母细胞瘤分化的关键调节因子。为了从分子层面理解HOXC9在促进神经母细胞瘤细胞分化中的功能，我们通过微阵列基因表达谱分析和染色质免疫沉淀结合大规模平行测序（ChIP-seq）对HOXC9诱导的分化程序进行了全基因组分析。在此，我们详细描述了与我们最近发表的论文[1]相关的微阵列和ChIP-seq数据生成的实验系统、方法和质量控制。
