Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, PR China.
Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, PR China.
Brain Behav Immun. 2014 Nov;42:212-21. doi: 10.1016/j.bbi.2014.06.202. Epub 2014 Jul 8.
Prenatal influenza virus infection has been associated with an increased risk of schizophrenia. Thus, inactivated flu vaccines are widely recommended for pregnant women. In a mouse model of pregnancy, immune activation via exposure to viruses or lipopolysaccharide (LPS) impaired brain development and behavioral function in offspring. The objective of our study was to determine if flu vaccination as an immune activation could affect postnatal neurogenesis and behavior. Female C57BL/6J mice were administered A(H1N1) influenza vaccine (AIV) or seasonal influenza vaccine (SIV) early in pregnancy. We found that the offspring of vaccinated mice, especially AIV group, presented superior performance in terms of exploratory behavior and spatial ability compared with controls at postnatal day 28 (P28), but at P56, there was no significance differences among these pups. Quantification of BrdU(+)/DCX(+) and BrdU(+)/NeuN(+) cells in the dentate gyrus (DG) indicated an increase in the hippocampal neurogenesis of the pups born to both vaccinated mothers. The cytokine levels in both the serum and hippocampus changed to varying degrees. Furthermore, administration of the A(H1N1) vaccine blocked LPS-induced cognitive impairment in the progeny. Altogether, the results suggest that maternal influenza vaccination promotes neurogenesis and behavioral function, as well as protection from LPS insults in the developing offspring.
产前流感病毒感染与精神分裂症风险增加有关。因此，灭活流感疫苗被广泛推荐给孕妇。在妊娠小鼠模型中，通过暴露于病毒或脂多糖（LPS）激活免疫系统会损害后代的大脑发育和行为功能。我们的研究目的是确定流感疫苗接种作为免疫激活是否会影响产后神经发生和行为。雌性 C57BL/6J 小鼠在妊娠早期接受 A(H1N1) 流感疫苗（AIV）或季节性流感疫苗（SIV）。我们发现，与对照组相比，接种疫苗的小鼠后代，尤其是 AIV 组，在出生后 28 天（P28）时表现出更好的探索行为和空间能力，但在 P56 时，这些幼崽之间没有差异。在齿状回（DG）中 BrdU(+)/DCX(+) 和 BrdU(+)/NeuN(+) 细胞的定量表明，来自接种疫苗母亲的幼崽的海马神经发生增加。血清和海马中的细胞因子水平也发生了不同程度的变化。此外，A(H1N1) 疫苗的给药阻断了 LPS 诱导的后代认知障碍。总之，这些结果表明，母体流感疫苗接种可促进神经发生和行为功能，并可预防发育中后代受到 LPS 的伤害。
