氧化铜纳米片增强流动注射化学发光体系用于水样、药物和人血清中万古霉素的测定。
CuO nanosheets-enhanced flow-injection chemiluminescence system for determination of vancomycin in water, pharmaceutical and human serum.
机构信息
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:737-43. doi: 10.1016/j.saa.2013.12.014. Epub 2013 Dec 12.
A novel, rapid and sensitive CuO nanosheets (NSs) amplified flow-injection chemiluminescence (CL) system, luminol-H2O2-CuO nanosheets, was developed for determination of the vancomycin hydrochloride for the first time. It was found that vancomycin could efficiently inhibit the CL intensity of luminol-H2O2-CuO nanosheets system in alkaline medium. Under the optimum conditions, the inhibited CL intensity was linearly proportional to the concentration of vancomycin over the ranges of 0.5-18.0 and 18.0-40.0 mg L(-1), with a detection limit (3σ) of 0.1 mg L(-1). The precision was calculated by analyzing samples containing 5.0 mg L(-1) vancomycin (n=11) and the relative standard deviation (RSD) was 2.8%. Also, a high injection throughput of 120 sample h(-1) was obtained. The CuO nanosheets were synthesized by a sonochemical method. Also, X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were employed to characterize the CuO nanosheets. The method was successfully employed to determine vancomycin hydrochloride in environmental water samples, pharmaceutical formulation and spiked human serum.
一种新型的、快速的、灵敏的氧化铜纳米片（NSs）放大流动注射化学发光（CL）系统，鲁米诺-H2O2-CuO 纳米片，被开发出来用于首次测定盐酸万古霉素。研究发现，盐酸万古霉素可以在碱性介质中有效地抑制鲁米诺-H2O2-CuO 纳米片系统的 CL 强度。在最佳条件下，抑制后的 CL 强度与盐酸万古霉素的浓度在 0.5-18.0 和 18.0-40.0mg/L 范围内呈线性关系，检测限（3σ）为 0.1mg/L。通过分析含有 5.0mg/L 盐酸万古霉素的样品（n=11）来计算精密度，相对标准偏差（RSD）为 2.8%。此外，还可以获得 120 个样品/h 的高注入通量。CuO 纳米片通过超声化学法合成。同时，采用 X 射线衍射（XRD）和扫描电子显微镜（SEM）分析对 CuO 纳米片进行了表征。该方法成功地用于测定环境水样、药物制剂和加标人血清中的盐酸万古霉素。
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