Lombardi G, Padhye A A, Standard P G, Kaufman L, Ajello L
Division of Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Med Vet Mycol. 1989;27(2):113-20.
Apophysomyces elegans and Saksenaea vasiformis frequently fail to sporulate on routine media used in clinical laboratories, thus delaying or preventing their specific identification. We have developed exoantigen test reagents capable of identifying non-sporulating isolates of A. elegans and S. vasiformis. A reagent containing three specific A. elegans precipitins was produced when A. elegans antiserum was absorbed with S. vasiformis. Antigenic analyses showed that at least two serotypes of S. vasiformis exist. Only six of 10 S. vasiformis isolates studied produced bands of identity with S. vasiformis (B-2190) (serotype 1) antiserum absorbed with S. vasiformis (B-2189) (serotype 2) antigens. The remaining four isolates, however, produced bands of identity with S. vasiformis B-2189 antiserum absorbed with B-2190 antigens. Antigenic differences among the isolates of the two groups could be correlated to some extent with the morphologic differences observed, especially with respect to the size of the sporangia and sporangiospores.
雅致阿波菲霉和瓶梗霉在临床实验室常用的常规培养基上常常无法形成孢子，从而延误或阻碍对它们的特异性鉴定。我们开发了能够鉴定不产孢的雅致阿波菲霉和瓶梗霉分离株的外抗原测试试剂。当雅致阿波菲霉抗血清用瓶梗霉吸收后，产生了一种含有三种特异性雅致阿波菲霉沉淀素的试剂。抗原分析表明，瓶梗霉至少存在两种血清型。在所研究的10株瓶梗霉分离株中，只有6株与用瓶梗霉（B - 2189）（血清型2）抗原吸收后的瓶梗霉（B - 2190）（血清型1）抗血清产生相同条带。然而，其余4株与用B - 2190抗原吸收后的瓶梗霉B - 2189抗血清产生相同条带。两组分离株之间的抗原差异在一定程度上与观察到的形态学差异相关，特别是关于孢子囊和孢子囊孢子的大小。
