Sekhon A S, Padhye A A, Standard P G, Kaufman L, Ajello L, Garg A K
National Reference Center for Human Mycotic Diseases, University of Alberta, Edmonton, Canada.
J Med Vet Mycol. 1990;28(1):59-66.
Dactylaria gallopava and Scolecobasidium constrictum were reduced to varietal status under the new combination of Dactylaria constricta (Abbott) Dixon et Salkin var. gallopava (Cooke) Salkin & Dixon, and D. constricta (Abbott) Dixon et Salkin var. constricta, primarily on the basis of the morphologic similarity of their two-celled, dematiaceous, blastic conidia. To appraise this taxonomic change, we studied the antigenic relationship of D. gallopava to S. constrictum using the exoantigen procedure. Exoantigens were prepared from 20 isolates of D. gallopava, seven isolates of S. constrictum and two isolates of S colecobasidium tschawytschae and were tested against reference rabbit anti-D. gallopava and anti-S. constrictum antisera in the presence of their homologous antigens using the micro-immunodiffusion technique. All D. gallopava isolates produced two to three distinct, identical exoantigens. The seven isolates of S. constrictum also produced two to three distinct exoantigens. None of the seven isolates of S. constrictum was reactive against the D. gallopava reference system. Three of the 20 D. gallopava culture filtrate antigens produced one or two precipitin bands of nonidentity with the S. constrictum reference reagents. Both isolates of S. tschawytschae were nonreactive with the D. gallopava and S. constrictum reference reagents. In addition, D. gallopava differed from S. constrictum in the production of a reddish-brown diffusible pigment, growth up to 45 degrees C, and sensitivity to cycloheximide. Based on these physiologic differences and little or no antigenic relatedness between D. gallopava and S. constrictum, we conclude that these two species should be retained as separate entities rather than be considered as varieties of a single species.
根据新的组合，疾驰弯孢霉（Dactylaria gallopava）和缢缩柱孢霉（Scolecobasidium constrictum）被降为变种地位，分别为缢缩弯孢霉（Dactylaria constricta (Abbott) Dixon et Salkin var. gallopava (Cooke) Salkin & Dixon）和缢缩弯孢霉（D. constricta (Abbott) Dixon et Salkin var. constricta），主要依据其双细胞、暗色、芽生分生孢子的形态相似性。为评估这一分类学变化，我们采用外抗原程序研究了疾驰弯孢霉与缢缩柱孢霉的抗原关系。从20株疾驰弯孢霉、7株缢缩柱孢霉和2株察氏柱孢霉（S colecobasidium tschawytschae）的分离物中制备外抗原，并使用微量免疫扩散技术，在其同源抗原存在的情况下，用兔抗疾驰弯孢霉和抗缢缩柱孢霉参考抗血清进行检测。所有疾驰弯孢霉分离物产生两到三种不同的、相同的外抗原。7株缢缩柱孢霉分离物也产生两到三种不同的外抗原。7株缢缩柱孢霉分离物中没有一株与疾驰弯孢霉参考系统发生反应。20株疾驰弯孢霉培养滤液抗原中的3株产生了一到两条与缢缩柱孢霉参考试剂不同的沉淀带。两株察氏柱孢霉分离物与疾驰弯孢霉和缢缩柱孢霉参考试剂均无反应。此外，疾驰弯孢霉在产生红棕色可扩散色素、在45摄氏度下生长以及对放线菌酮的敏感性方面与缢缩柱孢霉不同。基于这些生理差异以及疾驰弯孢霉和缢缩柱孢霉之间几乎没有或没有抗原相关性，我们得出结论，这两个物种应保留为独立的实体，而不应被视为单一物种的变种。
