Standard P G, Kaufman L, Padhye A A
Division of Mycotic Diseases, Centers for Disease Control, Atlanta, GA 30333.
Diagn Clin Immunol. 1988;5(6):367-70.
Phaeoannellomyces werneckii is identified on the basis of its annellidic conidiogenous cells and the production of two-celled conidia. Without the proper recognition of its annellidic mode of conidiogenesis, isolates of P. werneckii can be confused with other species of Phaeococcomyces, Exophiala, and Scolecobasidium constrictum. To overcome this problem, we developed exoantigen reagents capable of specifically identifying P. werneckii. Rabbit P. werneckii antiserum was rendered specific by adsorptions with antigens of Exophiala jeanselmei serotype 1, Exophiala moniliae, and Wangiella dermatitidis. Eighty-two dematiaceous yeast-like fungi, including 18 P. werneckii cultures, were tested in the immunodiffusion test against P. werneckii antisera. Exoantigen tests permitted the rapid and specific identification of the 18 P. werneckii isolates. Negative results were obtained with the 64 heterologous antigen extracts.
威尼克氏暗梗孢酵母是根据其瓶梗状分生孢子产孢细胞和双细胞分生孢子的产生来鉴定的。如果不能正确认识其瓶梗状分生孢子形成方式，威尼克氏暗梗孢酵母的分离株可能会与其他种类的暗色球腔菌属、外瓶霉属和缢缩梗柱孢属混淆。为克服这一问题，我们开发了能够特异性鉴定威尼克氏暗梗孢酵母的外抗原试剂。通过用1型甄氏外瓶霉、念珠状外瓶霉和皮炎万吉拉霉的抗原进行吸附处理，使兔抗威尼克氏暗梗孢酵母抗血清具有特异性。用82株暗色酵母菌样真菌（包括18株威尼克氏暗梗孢酵母培养物）针对威尼克氏暗梗孢酵母抗血清进行免疫扩散试验。外抗原试验能够快速、特异性地鉴定出这18株威尼克氏暗梗孢酵母分离株。64种异源抗原提取物的检测结果均为阴性。
