Reifschneider Olga, Schütz Christian L, Brochhausen Christoph, Hampel Gabriele, Ross Tobias, Sperling Michael, Karst Uwe
Anal Bioanal Chem. 2015 Mar;407(9):2365-71. doi: 10.1007/s00216-014-8012-4.
An analytical method using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to assess enrichment of 10B-containing p-boronophenylalanine-fructose (BPA-f) and its pharmacokinetic distribution in human tissues after application for boron neutron capture therapy (BNCT). High spatial resolution (50 μm) and limits of detection in the low parts-per-billion range were achieved using a Nd:YAG laser of 213 nm wavelength. External calibration by means of 10B-enriched standards based on whole blood proved to yield precise quantification results. Using this calibration method, quantification of 10B in cancerous and healthy tissue was carried out. Additionally, the distribution of 11B was investigated, providing 10B enrichment in the investigated tissues. Quantitative imaging of 10B by means of LA-ICP-MS was demonstrated as a new option to characterise the efficacy of boron compounds for BNCT.
开发了一种使用激光烧蚀-电感耦合等离子体质谱法（LA-ICP-MS）的分析方法，并将其应用于评估含硼-10的对硼苯丙氨酸-果糖（BPA-f）的富集情况及其在硼中子俘获疗法（BNCT）应用后在人体组织中的药代动力学分布。使用波长为213 nm的Nd:YAG激光实现了高空间分辨率（50μm）和低十亿分之一范围内的检测限。基于全血的硼-10富集标准进行外部校准，结果证明可产生精确的定量结果。使用这种校准方法，对癌组织和健康组织中的硼-10进行了定量。此外，还研究了硼-11的分布情况，以了解所研究组织中硼-10的富集情况。通过LA-ICP-MS对硼-10进行定量成像被证明是表征硼化合物用于BNCT疗效的一种新方法。
