Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Centre for Biology of Integrated Systems, Tallinn University of Technology, Akadeemia tee, Tallinn, Estonia.
Forensic Sci Int Genet. 2014 Sep;12:192-8. doi: 10.1016/j.fsigen.2014.05.014. Epub 2014 Jun 24.
Success of DNA typing is related to the amount of target material recovered from an evidentiary item. Generally, the more DNA that is recovered, the better the chance is of obtaining a typing result that will be robust and reliable. One method of collecting stain materials is by swabbing. Recovery of DNA from a number of commercially available swabs is not an efficient process. The X-Swab™ (Diomics Corporation, La Jolla, CA) is a unique bio-specimen collection material with highly absorptive properties and can be dissolved during certain extraction conditions. Therefore, more DNA may be collected from a substrate and be released from the swab matrix than other swabs. The ability to recover DNA from X-Swab material and success in STR typing were compared with the Copan 4N6FLOQSwab™ (Brescia, Italy), a device which utilizes a proprietary flocked-swab technology to maximize DNA collection and elution efficiency. Both types of swabs were impregnated with known amounts of DNA and body fluids and allowed to air dry. In addition, blood was placed onto glass slides, allowed to dry and collected using both types of swabs. DNA recovery was assessed by DNA quantitation and by STR typing. Results suggested that X-Swab material yielded greater DNA recovery, particularly of low quantity samples (defined as diluted neat samples), compared with the 4N6FLOQSwab. Results also indicated that X-Swab material itself enhances yield of PCR products.
DNA 分型的成功与从证据物品中回收的目标物质的量有关。通常，回收的 DNA 越多，获得可靠和稳健的分型结果的机会就越大。一种收集污渍材料的方法是擦拭。从许多市售的拭子中回收 DNA 不是一个有效的过程。X-Swab™（Diomics Corporation，加利福尼亚州拉霍亚）是一种具有高吸收性的独特生物标本收集材料，并可在某些提取条件下溶解。因此，与其他拭子相比，可能从基质中收集到更多的 DNA 并从拭子基质中释放出来。比较了从 X-Swab 材料中回收 DNA 的能力和 STR 分型的成功率，与利用专有植绒拭子技术最大限度地提高 DNA 收集和洗脱效率的 Copan 4N6FLOQSwab™（意大利布雷西亚）进行了比较。两种拭子都用已知量的 DNA 和体液浸渍，并风干。此外，将血液放在载玻片上，风干后使用两种拭子进行收集。通过 DNA 定量和 STR 分型评估 DNA 回收情况。结果表明，与 4N6FLOQSwab 相比，X-Swab 材料的 DNA 回收量更大，特别是在低数量样本（定义为稀释的纯样本）方面。结果还表明，X-Swab 材料本身可以提高 PCR 产物的产量。
