Revathi Peranandam, Iyapparaj Palanisamy, Vasanthi Lourduraj Arockia, Munuswamy Natesan, Prasanna Vimalanathan Arun, Pandiyarajan Jayaraj, Krishnan Muthukalingan
Department of Environmental Biotechnology, Bharathidasan University, Trichy, 620 024, Tamil Nadu, India,
Bull Environ Contam Toxicol. 2014 Oct;93(4):446-51. doi: 10.1007/s00128-014-1332-4. Epub 2014 Jul 14.
In the present study, the effect of tributyltin (TBT) on the histopathological and hormonal changes during spermatogenesis in freshwater prawn Macrobrachium rosenbergii was documented. Three experimental concentrations such as 10, 100 and 1,000 ng/L were selected and exposed to prawns for 45 days. After TBT exposure, the reproductive activities like sperm count and sperm length were decreased when compared with control. Further, abnormal structure of the seminiferous tubule, decrease in spermatozoa concentration, diminution of the seminiferous tubule membrane and the abundance of spermatocytes in the testis were noticed in treated prawns. Interestingly, radioimmunoassay clearly revealed the reduction of testosterone level in TBT exposed groups. Thus, TBT has considerably reduced the level of testosterone and caused the impairment of spermatogenesis in the freshwater male prawn M. rosenbergii.
在本研究中，记录了三丁基锡（TBT）对淡水罗氏沼虾精子发生过程中组织病理学和激素变化的影响。选择了10、100和1000 ng/L这三种实验浓度，并让虾暴露于其中45天。暴露于TBT后，与对照组相比，精子计数和精子长度等生殖活动减少。此外，在处理过的虾中发现了生精小管结构异常、精子浓度降低、生精小管膜减少以及睾丸中生精细胞数量增多的情况。有趣的是，放射免疫分析清楚地显示了TBT暴露组睾酮水平的降低。因此，TBT显著降低了睾酮水平，并导致淡水雄性罗氏沼虾精子发生受损。
