Moodley Kogi, Naidoo Yougasphree, Mackraj Irene
Department of Human Physiology, School of Basic Medical Sciences, Faculty of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
Department of Biology, School of Biological and Conservation Sciences, Faculty of Science and Agriculture, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
J Ethnopharmacol. 2014 Sep 11;155(2):1194-203. doi: 10.1016/j.jep.2014.07.003. Epub 2014 Jul 11.
Tulbaghia violacea has been used traditionally for the treatment of several ailments, including hypertension. The herb has been shown to have antihypertensive properties which have been attributed to its angiotensin-converting enzymeinhibitory (ACEI) activity. It could, therefore, prove beneficial in ameliorating renal pathology associated with hypertension. To evaluate the effects of long-term administration of Tulbaghia violacea on renal function and morphology in the Dahl salt-sensitive (DSS) rat model.
Male DSS rats were treated intra-peritoneally (i.p.) as follows: methanolic extract of Tulbaghia violacea: (TVL) (50 mg/kg/b.w.), captopril: (CAP) (25 mg/kg/b.w.), or distilled water, control: (CON) (3 ml/kg/b.w.). Blood pressure (BP) was measured bi-weekly, whilst 24-h urine volumes and electrolyte concentrations were assessed weekly. Animals were sacrificed on day 49 by halothane overdose. Blood was removed for determination of plasma and serum electrolytes. Left kidney tissues were harvested for the determination of nuclear factor-kappaβ (NF-kβ) and transforming growth factor-β (TGF-β) gene expressions.
TVL significantly reduced mean arterial pressure (MAP) and diastolic blood pressure (DBP). TVL showed reduced blood urea nitrogen, serum creatinine, total protein in urine as well as increased serum total protein. TVL decreased thiobarbituric acid reactive substances (TBARS) and increased glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity and nitric oxide significantly. NF-kβ and TGF-β) gene expressions were significantly reduced in TVL and CAP treated rats. Moreover, renal morphology improved significantly in TVL and CAP treated animals.
TVL and CAP demonstrated marked improvement in renal function and morphology.
紫花郁金香传统上用于治疗多种疾病，包括高血压。该草药已被证明具有降压特性，这归因于其血管紧张素转换酶抑制（ACEI）活性。因此，它可能有助于改善与高血压相关的肾脏病理。为了评估长期给予紫花郁金香对Dahl盐敏感（DSS）大鼠模型肾功能和形态的影响。
雄性DSS大鼠按以下方式腹腔注射（i.p.）：紫花郁金香甲醇提取物：（TVL）（50mg/kg体重），卡托普利：（CAP）（25mg/kg体重），或蒸馏水，对照组：（CON）（3ml/kg体重）。每两周测量一次血压（BP），同时每周评估24小时尿量和电解质浓度。在第49天通过过量给予氟烷处死动物。取血测定血浆和血清电解质。收集左肾组织测定核因子-κβ（NF-κβ）和转化生长因子-β（TGF-β）基因表达。
TVL显著降低平均动脉压（MAP）和舒张压（DBP）。TVL使血尿素氮、血清肌酐、尿总蛋白降低，血清总蛋白升高。TVL显著降低硫代巴比妥酸反应性物质（TBARS），提高谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性以及一氧化氮水平。在TVL和CAP处理的大鼠中，NF-κβ和TGF-β基因表达显著降低。此外，TVL和CAP处理的动物肾脏形态有显著改善。
TVL和CAP在肾功能和形态方面表现出显著改善。
