Hu Xiu-Feng, Yao Jun, Gao She-Gan, Yang Yan-Tong, Peng Xiu-Qing, Feng Xiao-Shan
Department of Oncology, Cancer Research Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Mol Med Rep. 2014 Sep;10(3):1409-15. doi: 10.3892/mmr.2014.2369. Epub 2014 Jul 9.
The present study aimed to determine whether the expression levels of midkine (MK) and syndecan‑1 correlate with the malignant progression and poor prognosis of gastric cardiac adenocarcinoma (GCA). GCA tissue samples (n=72) were obtained from the Department of Pathology of the First Affiliated Hospital of Henan University of Science and Technology (Luoyang, China). The paraffin‑embedded samples had been surgically resected and pathologically diagnosed between 2007 and 2009. Normal gastric cardiac biopsy specimens (n=40) were also collected as the control. The expression levels of MK and syndecan‑1 were assessed by immunohistochemistry using the high‑sensitivity streptavidin‑peroxidase method. Statistical analysis was performed on the data obtained using the SPSS 17.0 statistics package. MK expression was detected in 76.4% of GCA samples and 5% of normal gastric cardiac mucosa specimens. A significant positive correlation was observed between the expression levels of MK and the infiltrative depth of the tumor, the presence of lymph node metastasis and the prognosis of the patients (P<0.05). Syndecan‑1 expression was detected in 38.9% of GCA samples and 100% of normal gastric cardiac mucosa samples. The expression levels of syndecan‑1 were negatively correlated with the grade of differentiation, serosal membrane invasion, lymph node metastasis and the patient's prognosis (P<0.05). Notably, the expression levels of MK and syndecan‑1 were inversely correlated (r=‑0.352, P<0.01) in the GCA tissue samples. These results suggest that high expression levels of MK in GCA tissues may indicate a differentiation stage that is characteristic of malignancy, a late clinical stage and a poor prognosis, whereas increased syndecan‑1 levels may indicate a high degree of differentiation, an early clinical stage and a favorable prognosis. MK and syndecan‑1 may serve as important biomarkers for monitoring the development and progression of GCA.
本研究旨在确定中期因子（MK）和多功能蛋白聚糖-1的表达水平是否与贲门胃腺癌（GCA）的恶性进展及不良预后相关。GCA组织样本（n = 72）取自河南科技大学第一附属医院（中国洛阳）病理科。这些石蜡包埋样本于2007年至2009年期间经手术切除并进行病理诊断。还收集了40例正常贲门胃活检标本作为对照。采用高敏链霉亲和素-过氧化物酶法通过免疫组织化学评估MK和多功能蛋白聚糖-1的表达水平。使用SPSS 17.0统计软件包对所得数据进行统计学分析。在76.4%的GCA样本和5%的正常贲门胃黏膜标本中检测到MK表达。MK表达水平与肿瘤浸润深度、淋巴结转移情况及患者预后之间存在显著正相关（P<0.05）。在38.9%的GCA样本和100%的正常贲门胃黏膜样本中检测到多功能蛋白聚糖-1表达。多功能蛋白聚糖-1表达水平与分化程度、浆膜侵犯、淋巴结转移及患者预后呈负相关（P<0.05）。值得注意的是，在GCA组织样本中，MK和多功能蛋白聚糖-1的表达水平呈负相关（r = -0.352，P<0.01）。这些结果表明，GCA组织中MK的高表达水平可能表明具有恶性特征的分化阶段、临床晚期及不良预后，而多功能蛋白聚糖-1水平升高可能表明高分化程度、临床早期及良好预后。MK和多功能蛋白聚糖-1可能作为监测GCA发生发展的重要生物标志物。
