Okshevsky Mira, Meyer Rikke Louise
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
J Microbiol Methods. 2014 Oct;105:102-4. doi: 10.1016/j.mimet.2014.07.010. Epub 2014 Jul 11.
Here we determine an optimal technique for the visualization of extracellular DNA in bacterial biofilms using the fluorescent eDNA stain TOTO-1 and the counterstain SYTO 60. This technique allows for more sensitive eDNA visualization than other fluorescent staining methods currently in use.
在这里,我们使用荧光细胞外DNA染色剂TOTO-1和复染剂SYTO 60确定了一种用于可视化细菌生物膜中细胞外DNA的最佳技术。与目前使用的其他荧光染色方法相比,该技术能更灵敏地实现细胞外DNA的可视化。