He Yuxuan, Li Hongyan, He Jianhua, Zhao Xingxu
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China.
Anim Reprod Sci. 2014 Aug;148(3-4):260-6. doi: 10.1016/j.anireprosci.2014.06.019. Epub 2014 Jun 27.
The aim of the study was to determine the effects of heavy ion radiation (HIR) on ram sperm quality during 24h of in vitro liquid storage at 15°C, and identify the most appropriate dose which did not injure, but actually improved sperm quality and confirmed the relationship between highly expressed enolase and ram sperm quality during storage in vitro. Six Dorset ram (Ovis aries) semen pools from five mature and healthy rams were each divided into seven experimental groups with different doses of HIR (0, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5Gy) under the same experimental conditions. Sperm motility, viability, ATP content, and the gene and protein expression of enolase were measured at 24h of storage. Irradiated semen which had been stored for 24h, retained not only greater sperm motility, viability, and ATP content, but had greater enolase protein expression. This was evidenced by increased amounts of mRNA for this enzyme and amount of enolase protein as compared with semen from control rams, especially for the 0.1Gy group (P<0.001). These results indicate that HIR can promote enhanced motility and viability during in vitro liquid storage, and the 0.1Gy may be a suitable dose for improving sperm quality. Greater amounts of enolase and ATP content may results from enhanced sperm glycolysis by HIR. HIR enhances sperm glycolysis to generate sufficient ATP for maintaining sperm motility during storage.
本研究的目的是确定重离子辐射（HIR）对15°C体外液体保存24小时期间公羊精子质量的影响，确定最适宜且不会损伤反而能改善精子质量的剂量，并证实体外保存期间烯醇化酶高表达与公羊精子质量之间的关系。将来自5只成熟健康公羊的6个多塞特公羊精液池在相同实验条件下分为7个不同HIR剂量（0、0.05、0.1、0.2、0.3、0.4和0.5Gy）的实验组。在保存24小时时测量精子活力、存活率、ATP含量以及烯醇化酶的基因和蛋白表达。储存24小时的辐照精液不仅保持了更高的精子活力、存活率和ATP含量，而且烯醇化酶蛋白表达更高。与对照公羊的精液相比，该酶的mRNA量和烯醇化酶蛋白量增加，尤其是0.1Gy组（P<0.001），证明了这一点。这些结果表明，HIR可促进体外液体保存期间精子活力和存活率的提高，0.1Gy可能是改善精子质量的适宜剂量。HIR可能通过增强精子糖酵解作用导致烯醇化酶和ATP含量增加。HIR增强精子糖酵解作用以产生足够的ATP来维持储存期间的精子活力。
