Egr-1通过启动子中一个Sp1/Egr-1重叠位点调控NGX6基因的转录。
Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter.
作者信息
Liu Minji, Wang Xiaoyan, Peng Ya, Shen Shourong, Li Guiyuan
机构信息
Department of Gastroenterology, Zhuzhou central Hospital, 412007 Zhuzhou, Hunan, China.
出版信息
BMC Mol Biol. 2014 Jul 16;15:14. doi: 10.1186/1471-2199-15-14.
BACKGROUND
As a novel candidate metastasis suppressor gene, Nasopharyngeal carcinoma-associated gene 6 (NGX6) is involved in cellular growth, cell cycle progression and tumor angiogenesis. Previous studies have shown that NGX6 gene is down-regulated in colorectal cancer (CRC). However, little is known about its transcriptional regulation.
RESULTS
We defined the minimal promoter of NGX6 gene in a 186-bp region (from-86 to +100) through mutation construct methods and luciferase assays. Results from Electrophoretic mobility shift assays (EMSA) and Chromatin immunoprecipitation (ChIP) revealed that Early growth response gene 1 (Egr-1) binds to the Sp1/Egr-1 overlapping site of NGX6 minimal promoter. Overexpression of Egr-1 increased the promoter activity and mRNA level of NGX6 gene; while knock-down of endogenous Egr-1 decreased mRNA expression of NGX6 gene.
CONCLUSION
These results demonstrate that Egr-1 regulates NGX6 gene transcription through an overlapping Sp1/Egr-1 binding site as a positive regulator of NGX6 gene.
背景
作为一种新型的潜在转移抑制基因，鼻咽癌相关基因6（NGX6）参与细胞生长、细胞周期进程及肿瘤血管生成。先前研究表明，NGX6基因在结直肠癌（CRC）中表达下调。然而，其转录调控机制尚不清楚。
结果
我们通过突变构建方法和荧光素酶检测，在一个186bp区域（从-86至+100）定义了NGX6基因的最小启动子。电泳迁移率变动分析（EMSA）和染色质免疫沉淀（ChIP）结果显示，早期生长反应基因1（Egr-1）与NGX6最小启动子的Sp1/Egr-1重叠位点结合。Egr-1过表达增加了NGX6基因的启动子活性和mRNA水平；而敲低内源性Egr-1则降低了NGX6基因的mRNA表达。
结论
这些结果表明，Egr-1作为NGX6基因的正向调节因子，通过一个重叠的Sp1/Egr-1结合位点调控NGX6基因转录。
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