Qadri F, Haq S, Ciznár I
Laboratory Sciences Division, International Centre for Diarrhoeal Disease and Research, Bangladesh.
Infect Immun. 1989 Sep;57(9):2909-11. doi: 10.1128/iai.57.9.2909-2911.1989.
Strains of Shigella dysenteriae type 1 cultured in Casamino Acids-yeast extract broth medium in the presence of 1 mM calcium chloride at 37 degrees C for 22 h induced hemagglutination of erythrocytes that was inhibited by N-acetylneuraminic acid, N-acetylneuramin-lactose, and alpha 1-glycoprotein. The hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). Under the same conditions, strains of Shigella flexneri (types 1a, 1b, 2a, and 2b) showed N-acetylneuraminic acid-resistant hemagglutination of erythrocytes.
在含有1 mM氯化钙的酪蛋白氨基酸 - 酵母提取物肉汤培养基中,于37℃培养22小时的1型痢疾志贺菌菌株可诱导红细胞发生血凝反应,该反应可被N - 乙酰神经氨酸、N - 乙酰神经氨酸乳糖和α1 - 糖蛋白抑制。血凝反应对热不稳定,且细胞表面不存在附属物提示存在一种非菌毛黏附素。在相同条件下,福氏志贺菌菌株(1a、1b、2a和2b型)表现出对红细胞的N - 乙酰神经氨酸抗性血凝反应。