Qadri F, Haque A, Hossain A, Albert M J
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Microbiol Immunol. 1994;38(1):11-8. doi: 10.1111/j.1348-0421.1994.tb01738.x.
Electron microscopy of ruthenium red-stained ultrathin section of strains of Shigella dysenteriae type 1 grown in the Casamino Acids-yeast extract broth medium showed the presence of an extracellular slime layer. The slime appeared as a dense sheath covering bacteria. The presence of slime promoted hemagglutinating activity of the bacteria. The slime polysaccharide (SPS) isolated from the cell-free culture supernatant or the bacterial surface was less than 162,000 daltons in size and immunochemically similar. The SPS showed cross-reaction with lipopolysaccharide (LPS) antigen in immunological tests; however, it also appeared to be different from LPS since it did not contain 2-keto-3-deoxyoctonate, a core sugar of LPS. A different pattern of separation from LPS was also observed by silver staining of SDS-polyacrylamide gels. From these data it appeared that either LPS and SPS are contaminated with each other or that SPS is the polysaccharide portion of LPS.
对在酪蛋白氨基酸 - 酵母提取物肉汤培养基中生长的1型痢疾志贺氏菌菌株进行钌红染色超薄切片的电子显微镜观察显示，存在细胞外黏液层。该黏液表现为覆盖细菌的致密鞘。黏液的存在促进了细菌的血凝活性。从无细胞培养上清液或细菌表面分离的黏液多糖（SPS）大小小于162,000道尔顿，且免疫化学性质相似。在免疫试验中，SPS与脂多糖（LPS）抗原发生交叉反应；然而，它似乎也与LPS不同，因为它不含LPS的核心糖2 - 酮 - 3 - 脱氧辛酸。通过SDS - 聚丙烯酰胺凝胶的银染色也观察到与LPS不同的分离模式。从这些数据来看，似乎LPS和SPS相互污染，或者SPS是LPS的多糖部分。
